Integrin alpha 9 Antibody (3E4) - Azide and BSA Free

Western Blot: Integrin alpha 9 Antibody (3E4) [H00003680-M01] - ITGA9 monoclonal antibody (M01), clone 3E4. Analysis of ITGA9 expression in HepG2.

• Reactivity: Hu
• Applications: WB, ELISA, ICC/IF, IHC, IP
• Clone: 3E4
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free

Integrin alpha 9 Antibody (3E4) - Azide and BSA Free Summary

• Description: Quality control test: Antibody Reactive Against Recombinant Protein.
• Immunogen: ITGA9 (NP_002198, 785 a.a. ~ 886 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. GESVDAANFIQLDDLECHFQPINITLQVYNTGPSTLPGSSVSISFPNRLSSGGAEMFHVQEMVVGQEKGNCSFQKNPTPCIIPQEQENIFHTIFAFFTKSGR
• Specificity: ITGA9 - integrin, alpha 9
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: ITGA9
• Purity: IgG purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Immunoprecipitation
  • Western Blot 1:500
• Application Notes: Antibody reactivity against Recombinant Protein with GST tag on ELISA and WB. GST tag alone is used as a negative control.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: In 1x PBS, pH 7.4
• Preservative: No Preservative
• Purity: IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for Integrin alpha 9 Antibody (3E4) - Azide and BSA Free

• ALPHA-RLC
• Integrin alpha 9
• integrin alpha-9
• Integrin alpha-RLC
• integrin, alpha 9
• ITGA4L
• ITGA9
• RLC
• RLC-alpha

Background

This gene encodes an alpha integrin. Integrins are heterodimeric integral membrane glycoproteins composed of an alpha chain and a beta chain that mediate cell-cell and cell-matrix adhesion. The protein encoded by this gene, when bound to the beta 1 chain, forms an integrin that is a receptor for VCAM1, cytotactin and osteopontin. Expression of this gene has been found to be upregulated in small cell lung cancers.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Integrin alpha 9 Antibody (H00003680-M01)(6)

Publications using H00003680-M01

• Natalia N, Carla M, Julia S et al. Integrin alpha9 emerges as a key therapeutic target to reduce metastasis in rhabdomyosarcoma and neuroblastoma. Cell Mol Life Sci. 2022-10-11 [PMID: 36221013]
• Molist C, Navarro N, Giralt I et al. miRNA-7 and miRNA-324-5p regulate alpha9-Integrin expression and exert anti-oncogenic effects in rhabdomyosarcoma. Cancer Lett. 2020-03-03 [PMID: 32142919]
• Masia A, Almazan-Moga A, Velasco P et al. Notch-mediated induction of N-cadherin and alpha9-integrin confers higher invasive phenotype on rhabdomyosarcoma cells. Br J Cancer. 2012-09-13 [PMID: 22976797]
• Zeyda M, Gollinger K, Todoric J et al. Osteopontin Is an Activator of Human Adipose Tissue Macrophages and Directly Affects Adipocyte Function. Endocrinology. 2011-04-05 [PMID: 21467192]
• Grassinger J, Haylock DN, Storan MJ et al. Thrombin cleaved osteopontin regulates hemopoietic stem and progenitor cell functions through interactions with {alpha}9{beta}1 and {alpha}4{beta}1 integrins. Blood. 2009-05-05 [PMID: 19417209]
• Meyer C, Brieger A, Plotz G et al. An Interstitial Deletion at 3p21.3 Results in the Genetic Fusion of MLH1 and ITGA9 in a Lynch Syndrome Family. Clin Cancer Res. 2009-02-01 [PMID: 19188145]

Bioinformatics

• Gene Symbol: ITGA9
• Entrez:
  • 3680(Human)
• OMIM:
  • 603963(Human)
• Uniprot:
  • NP_002198()