Zyxin Antibody

Western Blot: Zyxin Antibody [NBP1-46135] - (0.1ug/ml) staining of Peripheral Blood Mononucleocytes lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

• Reactivity: Hu, Ca
• Applications: WB, ELISA
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

Zyxin Antibody Summary

• Immunogen: Peptide with sequence C-QQREKPRVQEKQH corresponding to internal region according to NP_003452.1.
• Epitope: internal region
• Marker: Focal Adhesion Marker
• Specificity: Reported variants represent identical protein: NP_003452.1, NP_001010972.1
• Predicted Species: Canine (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: ZYX
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Peptide ELISA Detection limit 1:4000
  • Western Blot 0.1 - 0.3 ug/ml
• Application Notes: WB: Approx. 85 kDa band observed in human peripheral blood mononucleocytes lysates (calculated MW of 61.3 kDa band according to NP_003452.1). The observed molecular weight corresponds to earlier findings with different antibodies from other sources.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for Zyxin Antibody

• CTCL tumor antigen se14-3
• CTCL-associated antigen se14-3
• cutaneous T-cell lymphoma associated antigen se14-3
• Cutaneous T-cell lymphoma-associated antigen se14-3
• KIAA1125
• MGC31836
• predicted protein of HQ2893
• PRKCBP1
• PRO2893
• protein kinase C-binding protein 1
• Rack7
• RACK7protein kinase C binding protein 1
• zinc finger MYND domain containing protein 8
• Zinc finger MYND domain-containing protein 8
• zinc finger, MYND-type containing 8
• Zyx
• Zyxin 2
• Zyxin

Background

Focal adhesions are actin-rich structures that enable cells to adhere to the extracellular matrix and at which protein complexes involved in signal transduction assemble. Zyxin is a zinc-binding phosphoprotein that concentrates at focal adhesions and along the actin cytoskeleton. Zyxin has an N-terminal proline-rich domain and three LIM domains in its C-terminal half. The proline-rich domain may interact with SH3 domains of proteins involved in signal transduction pathways while the LIM domains are likely involved in protein-protein binding. Zyxin may function as a messenger in the signal transduction pathway that mediates adhesion-stimulated changes in gene expression and may modulate the cytoskeletal organization of actin bundles. Alternative splicing results in multiple transcript variants that encode the same isoform.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Zyxin Antibody (NBP1-46135)(1)

Publication using NBP1-46135

• Li B, Zhuang L, Trueb B. Zyxin interacts with the SH3 domains of the cytoskeletal proteins LIM-nebulette and Lasp-1. J Biol Chem 2004-05-01 [PMID: 15004028]

Bioinformatics

• Gene Symbol: ZYX
• Entrez:
  • 7791(Human)
• Uniprot:
  • NP_003452.1(Human)