Zyxin Antibody (2D1) - Azide and BSA Free

Western Blot: Zyxin Antibody (2D1) [H00007791-M02] - ZYX monoclonal antibody (M02), clone 2D1 Analysis of ZYX expression in NIH/3T3.

Immunohistochemistry-Paraffin: Zyxin Antibody (2D1) [H00007791-M02] - Analysis of monoclonal antibody to ZYX on formalin-fixed paraffin-embedded human spleen. Antibody concentration 1.5 ug/ml.

Sandwich ELISA: Zyxin Antibody (2D1) [H00007791-M02] - Detection limit for recombinant GST tagged ZYX is 0.3 ng/ml as a capture antibody.

• Reactivity: Hu, Mu
• Applications: WB, ELISA, IHC
• Clone: 2D1
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free

Zyxin Antibody (2D1) - Azide and BSA Free Summary

• Description: Quality control test: Antibody Reactive Against Recombinant Protein.
• Immunogen: ZYX (AAH08743, 1 a.a. ~ 572 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MAAPRPSPAISVSVSAPAFYAPQEKFGPVVAPKPKVNPFRPGDSEPPPAPGAQRAQMGRVGEIPPPPPEDFPLPPPPLAGDGDDAEGALGGAFPPPPPPIEESFPPAPLEEEIFPSPPPPPEEEGGPEAPIPPPPQPREKVSSIDLEIDSLSSLLDDMTKNDPFKARVSSGYVPPPVATPFSSKSSTKPAAGGTAPLPPWKSPSSSQPLPQVPAPAQSQTQFHVQPQPQPKPQVQLHVQSQTQPVSLANTQPRGPPASSPAPAPKFSPVTPKFTPVASKFSPGAPGGSGSQPNQKLGHPEALSAGTGSPQPPSFTYAQQREKPRVQEKQHPVPPPAQNQNQVRSPGAPGPLTLKEVEELEQLTQQLMQDMEHPQRQNVAVNELCGRCHQPLARAQPAVRALGQLFHIACFTCHQCAQQLQGQQFYSLEGAPYCEGCYTDTLEKCNTCGEPITDRMLRATGKAYHPHCFTCVVCARPLEGTSFIVDQANRPHCVPDYHKQYAPRCSVCSEPIMPEPGRDETVRVVALDKNFHMKCYECEDCGKPLSIEADDNGCFPLDGHVLCRKCHTARAQT
• Marker: Focal Adhesion Marker
• Specificity: ZYX (2D1)
• Isotype: IgG2a Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: ZYX
• Purity: IgG purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Sandwich ELISA
  • Western Blot
• Application Notes: Antibody reactivity against cell lysate and recombinant protein for WB. It has also been used for ELISA and IHC-P.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: In 1x PBS, pH 7.4
• Preservative: No Preservative
• Purity: IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for Zyxin Antibody (2D1) - Azide and BSA Free

• CTCL tumor antigen se14-3
• CTCL-associated antigen se14-3
• cutaneous T-cell lymphoma associated antigen se14-3
• Cutaneous T-cell lymphoma-associated antigen se14-3
• KIAA1125
• MGC31836
• predicted protein of HQ2893
• PRKCBP1
• PRO2893
• protein kinase C-binding protein 1
• Rack7
• RACK7protein kinase C binding protein 1
• zinc finger MYND domain containing protein 8
• Zinc finger MYND domain-containing protein 8
• zinc finger, MYND-type containing 8
• Zyx
• Zyxin 2
• Zyxin

Background

Focal adhesions are actin-rich structures that enable cells to adhere to the extracellular matrix and at which protein complexes involved in signal transduction assemble. Zyxin is a zinc-binding phosphoprotein that concentrates at focal adhesions and along the actin cytoskeleton. Zyxin has an N-terminal proline-rich domain and three LIM domains in its C-terminal half. The proline-rich domain may interact with SH3 domains of proteins involved in signal transduction pathways while the LIM domains are likely involved in protein-protein binding. Zyxin may function as a messenger in the signal transduction pathway that mediates adhesion-stimulated changes in gene expression and may modulate the cytoskeletal organization of actin bundles. Alternative splicing results in multiple transcript variants that encode the same isoform.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Zyxin Antibody (H00007791-M02)(1)

Publication using H00007791-M02

• Mise N, Savai R, Yu H et al. Zyxin is a TGF-b/Smad3 target gene that regulates lung cancer cell motility via integrin alpha5b1. J Biol Chem. 2012-07-09 [PMID: 22778267]

Bioinformatics

• Gene Symbol: ZYX
• Entrez:
  • 7791(Human)
• OMIM:
  • 602002(Human)
• Uniprot:
  • AAH08743(Human)