ZWINT Antibody (6D3) - Azide and BSA Free

Western Blot: ZWINT Antibody (6D3) [H00011130-M01] - ZWINT monoclonal antibody (M01), clone 6D3 Analysis of ZWINT expression in Hela S3 NE.

Immunocytochemistry/Immunofluorescence: ZWINT Antibody (6D3) [H00011130-M01] - Analysis of monoclonal antibody to ZWINT on HeLa cell. Antibody concentration 10 ug/ml.

Sandwich ELISA: ZWINT Antibody (6D3) [H00011130-M01] - Detection limit for recombinant GST tagged ZWINT is approximately 0.03ng/ml as a capture antibody.

• Reactivity: Hu
• Applications: WB, ELISA, ICC/IF
• Clone: 6D3
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free

ZWINT Antibody (6D3) - Azide and BSA Free Summary

• Immunogen: ZWINT (AAH20979, 1 a.a. ~ 277 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MEAAETEAEAAALEVLAEVAGILEPVGLQEEAELPAKILVEFVVDSQKKDKLLCSQLQVADFLQNILAQEDTAKGLDPLASEDTSRQKAIAAKEQWKELKATYREHVEAIKIGLTKALTQMEEAQRKRTQLREAFEQLQAKKQMAMEKRRAVQNQWQLQQEKHLQHLAEVSAEVRERKTGTQQELDGVFQKLGNLKQQAEQERDKLQRYQTFLQLLYTLQGKLLFPEAEAEAENLPDDKPQQPTRPQEQSTGDTMGRDPGVSFKAVGLQPAGDVNLP
• Specificity: ZWINT - ZW10 interactor
• Isotype: IgG2a Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: ZWINT
• Purity: IgG purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunocytochemistry/ Immunofluorescence
  • Sandwich ELISA
  • Western Blot 1:500
• Application Notes: Antibody reactive against cell lysate and recombinant protein for Western Blot. Has also been used for immunofluoresence and ELISA.

Reactivity Notes

Human. Other species not tested.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: In 1x PBS, pH 7.4
• Preservative: No Preservative
• Purity: IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for ZWINT Antibody (6D3) - Azide and BSA Free

• C20orf164
• chromosome 20 open reading frame 164
• dJ337O18.7
• zinc finger SWIM domain-containing protein 3
• zinc finger, SWIM domain containing 3
• zinc finger, SWIM-type containing 3

Background

This gene encodes a protein that is clearly involved in kinetochore function although an exact role is not known. It interacts with ZW10, another kinetochore protein, possibly regulating the association between ZW10 and kinetochores. The encoded protein localizes to prophase kinetochores before ZW10 does and it remains detectable on the kinetochore until late anaphase. It has a uniform distribution in the cytoplasm of interphase cells. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: ZWINT
• Entrez:
  • 11130(Human)
• OMIM:
  • 609177(Human)
• Uniprot:
  • AAH20979(Human)