![Western Blot: ZWILCH Antibody (1C9) [H00055055-M01] - Analysis of ZWILCH expression in transfected 293T cell line by ZWILCH monoclonal antibody (M01), clone 1C9.Lane 1: ZWILCH transfected lysate(54.224 KDa).Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/ZWILCH-Antibody-1C9-Western-Blot-H00055055-M01-img0004.jpg "Western Blot: ZWILCH Antibody (1C9) [H00055055-M01] - Analysis of ZWILCH expression in transfected 293T cell line by ZWILCH monoclonal antibody (M01), clone 1C9.Lane 1: ZWILCH transfected lysate(54.224 KDa).Lane 2: Non-transfected lysate.")
| Reactivity | HuSpecies Glossary |
| Applications | WB, ELISA |
| Clone | 1C9 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Format | Azide and BSA Free |
| Description | Novus Biologicals Mouse ZWILCH Antibody (1C9) - Azide and BSA Free (H00055055-M01) is a monoclonal antibody validated for use in WB and ELISA. All Novus Biologicals antibodies are covered by our 100% guarantee. |
| Immunogen | ZWILCH (AAH36900, 1 a.a. ~ 477 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MAHNPNMTHLKINLPVTALPPLWVRCDSSDPEGTCWLGAELITTNNSITGIVLYVVSCKADKNYSVNLENLKNLHKKRHHLSTVTSKGFAQYELFKSSALDDTITASQTAIALDISWSPVDEILQIPPLSSTATLNIKVESGEPRGPLNHLYRELKFLLVLADGLRTGVTEWLEPLEAKSAVELVQEFLNDLNKLDGFGDSTKKDTEVETLKHDTAAVDRSVKRLFKVRSDLDFAEQLWCKMSSSVISYQDLVKCFTLIIQSLQRGDIQPWLHSGSNSLLSKLIHQSYHGTMDTVSLSGTIPVQMLLEIGLDKLKKDYISFFIGQELASLNHLEYFIAPSVDIQEQVYRVQKLHHILEILVSCMPFIKSQHELLFSLTQICIKYYKQNPLDEQHIFQLPVRPTAVKNLYQSEKPQKWRVEIYSGQKKIKTVWQLSDSSPIDHLNFHKPDFSELTLNGSLEERIFFTNMVTCSQVHFK |
| Localization | kinetochore |
| Specificity | ZWILCH - Zwilch |
| Isotype | IgG1 Kappa |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | ZWILCH |
| Purity | IgG purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | Antibody reactive against transfected lysate and recombinant protein for western blot. It has also been used for ELISA. |
| Storage | Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles. |
| Buffer | In 1x PBS, pH 7.4 |
| Preservative | No Preservative |
| Purity | IgG purified |
Secondary Antibodies |
Isotype Controls |
Research Areas for ZWILCH Antibody (H00055055-M01)Find related products by research area.
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![Sandwich ELISA: ZWILCH Antibody (1C9) [H00055055-M01] - Detection limit for recombinant GST tagged ZWILCH is approximately 1ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/ZWILCH-Antibody-1C9-Sandwich-ELISA-H00055055-M01-img0003.jpg "Sandwich ELISA: ZWILCH Antibody (1C9) [H00055055-M01] - Detection limit for recombinant GST tagged ZWILCH is approximately 1ng/ml as a capture antibody.")
![Immunocytochemistry Mxi1 Antibody [Unconjugated]](https://images.novusbio.com/images2/Mxi1_AF4185_Immunocytochemistry__Immunofluorescence_20286.jpg)
![Flow Cytometry: Mouse IgG1 Kappa Isotype Control (P3.6.2.8.1) [NBP1-43319] - Analysis of Alexa Fluor (R) 700 conjugate of NBP1-43319. Human buffy coat stained with mouse IgG1 Kappa isotype control. Flow cytometry image submitted by a verified customer review.](https://images.novusbio.com/images/Mouse-IgG1-Kappa-Light-Chain-Isotype-Control-P3-6-2-8-1-Flow-Cytometry-NBP1-43319-img0002.jpg "Flow Cytometry: Mouse IgG1 Kappa Isotype Control (P3.6.2.8.1) [NBP1-43319] - Analysis of Alexa Fluor (R) 700 conjugate of NBP1-43319. Human buffy coat stained with mouse IgG1 Kappa isotype control. Flow cytometry image submitted by a verified customer review.")