ZSCAN4 Antibody

Images

 
Western Blot: ZSCAN4 Antibody [NBP1-77120] - Mouse lung tissue lysate with ZSCAN4 antibody in (A) the absence and (B) presence of peptide blocking.
Immunocytochemistry/ Immunofluorescence: ZSCAN4 Antibody [NBP1-77120] - Rat Lung cells
Immunohistochemistry-Paraffin: ZSCAN4 Antibody [NBP1-77120] - Rat lung tissue with ZSCAN4 antibody at 5 ug/mL.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ELISA, ICC/IF, IHC, IHC-P, IF
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1 mg/ml

Order Details

ZSCAN4 Antibody Summary

Immunogen
Antibody was raised against an 18 amino acid synthetic peptide near the carboxy terminus of human ZSCAN4. The immunogen is located within amino acids 370 - 420 of ZSCAN4.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
ZSCAN4
Purity
Immunogen affinity purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Western Blot 1:100-1:2000
  • ELISA 1:100-1:2000
  • Immunocytochemistry/Immunofluorescence 20 ug/mL
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
  • Immunofluorescence
Theoretical MW
64 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Control Peptide
ZSCAN4 Peptide (NBP1-77120PEP)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for ZSCAN4 Antibody

  • FLJ35105
  • MGC126787
  • zinc finger and SCAN domain containing 4
  • Zinc finger protein 494MGC126789
  • ZNF494zinc finger and SCAN domain-containing protein 4

Background

Zytotic genome activation (ZGA) is the first and most critical transition event from the maternal genetic to the embryonic genetic program and the major burst of ZGA occurs only in the late 2-cell stage. ZSCAN4 encodes a SCAN domain and four zinc finger domains and is expressed in late 2-cell embryos and embryonic stem cells. Loss-of-function studies using siRNA shows delays in the progression from the 2-cell to the 4-cell stage and subsequent embryo development. ZSCAN4 plays an important role in the preimplantation development. Also, ZSCAN4 is involved in telomere maintenance and long term genomic stability in ES cells, suggesting that ZSCAN4 may be of critical importance in maintaining pluripotency in replicating stem cells.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol ZSCAN4
Uniprot