ZSCAN4 Antibody - BSA Free

Western Blot: ZSCAN4 Antibody [NBP1-77120] - Mouse lung tissue lysate with ZSCAN4 antibody in (A) the absence and (B) presence of peptide blocking.

Immunohistochemistry-Paraffin: ZSCAN4 Antibody [NBP1-77120] - Rat lung tissue with ZSCAN4 antibody at 5 ug/mL.

Immunocytochemistry/ Immunofluorescence: ZSCAN4 Antibody - BSA Free [NBP1-77120] - Immunofluorescence of ZSCAN4 in Rat Lung tissue with ZSCAN4 antibody at 20 ug/mL.

• Reactivity: Hu, Mu, Rt
• Applications: WB, ELISA, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

ZSCAN4 Antibody - BSA Free Summary

• Immunogen: Antibody was raised against an 18 amino acid synthetic peptide near the carboxy terminus of human ZSCAN4. The immunogen is located within amino acids 370 - 420 of ZSCAN4. Amino Acid Squence: HKTNLRSHERIHTGEKPY
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: ZSCAN4
• Purity: Peptide affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:100-1:2000
  • Immunocytochemistry/ Immunofluorescence 20 ug/mL
  • Immunohistochemistry 5 ug/ml
  • Immunohistochemistry-Paraffin 5 ug/ml
  • Western Blot 1:100-1:2000
• Theoretical MW: 64 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Control Peptide:

  ZSCAN4 Peptide (NBP1-77120PEP)

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Bovine (81%), Chicken (86%).

Packaging, Storage & Formulations

• Storage: Store at 4C.
• Buffer: PBS
• Preservative: 0.02% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Peptide affinity purified

Alternate Names for ZSCAN4 Antibody - BSA Free

• FLJ35105
• MGC126787
• zinc finger and SCAN domain containing 4
• Zinc finger protein 494MGC126789
• ZNF494zinc finger and SCAN domain-containing protein 4

Background

Zytotic genome activation (ZGA) is the first and most critical transition event from the maternal genetic to the embryonic genetic program and the major burst of ZGA occurs only in the late 2-cell stage. ZSCAN4 encodes a SCAN domain and four zinc finger domains and is expressed in late 2-cell embryos and embryonic stem cells. Loss-of-function studies using siRNA shows delays in the progression from the 2-cell to the 4-cell stage and subsequent embryo development. ZSCAN4 plays an important role in the preimplantation development. Also, ZSCAN4 is involved in telomere maintenance and long term genomic stability in ES cells, suggesting that ZSCAN4 may be of critical importance in maintaining pluripotency in replicating stem cells.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for ZSCAN4 Antibody (NBP1-77120)(1)

Publication using NBP1-77120

• Yu X, Liang S, Chen M et al. Recapitulating early human development with 8C-like cells Cell reports 2022-06-21 [PMID: 35732112] (ICC/IF, Human)

Bioinformatics

• Gene Symbol: ZSCAN4
• Uniprot:
  • NP_689890(Human)