ZSCAN21/ZFP38 Antibody

Western blot shows lysates of Caki-2 human clear cell carcinoma epithelial cell line, COLO 205 human colorectal adenocarcinoma cell line, and NTera-2 human testicular embryonic carcinoma cell line. PVDF membrane was probed with 2 µg/mL of Sheep Anti-Human ZSCAN21 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF8015) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for ZSCAN21 at approximately 60 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

• Reactivity: Hu
• Applications: WB
• Clonality: Polyclonal
• Host: Sheep
• Conjugate: Unconjugated
• Concentration: LYOPH

ZSCAN21/ZFP38 Antibody Summary

• Immunogen: E. coli-derived recombinant human ZSCAN21
  Asn139-Asp223
  Accession # Q9Y5A6
• Specificity: Detects human ZSCAN21 in direct ELISAs and Western blots.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Sheep
• Gene: ZSCAN21
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Western Blot 2 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Sterile PBS to a final concentration of 0.2 mg/mL.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for ZSCAN21/ZFP38 Antibody

• DKFZp434L134
• KOX25
• NY-REN-21
• Renal carcinoma antigen NY-REN-21
• ZFP38
• zfp-38
• ZFP383
• zinc finger and SCAN domain containing 21
• zinc finger and SCAN domain-containing protein 21
• zinc finger protein 38 (KOX 25)
• Zinc finger protein 38 homolog
• zinc finger protein 38
• zinc finger protein NY-REN-21 antigen
• Zipro1
• ZNF38
• ZNF38DKFZp686H10254
• ZSCAN21

Background

ZSCAN21 (Zinc finger and SCAN domain-containing protein 21; also Zfp-38, NY-REN-21, Zipro1 and Ru49) is a nuclear member of the multi-finger SCAN box protein family. It is expressed in select cell types, including cerebellar granule cells, Schwann cells, cerebral cortical and sympathetic neurons, round spermatids, and cells of the hair follicles plus sebaceous glands. It may have multiple functions, one of which is to initiate transcription of neuronal alpha -synuclein, while a second may be to promote granule cell proliferation. Human ZSCAN21 is 473 amino acids (aa) in length. It contains one SCAN (SREZBP, Ctfin51, AW-1/ ZNF174, and Number 18) domain (aa 45-127) that mediates dimerization, followed by seven consecutive C2H2-type zinc-finger regions (aa 277-466). ZSCAN21 is known to form both noncovalent homodimers and heterodimers with SCAND1. There is one potential isoform variant that shows a 188 aa substitution for aa 263-473. Over aa 139-223, human ZSCAN21 shares 54% aa sequence identity with mouse ZSCAN21.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: ZSCAN21
• Uniprot:
  • Q9Y5A6()