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SCAND1 Antibody

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Western Blot: SCAND1 Antibody [NBP2-56092] - Western blot analysis in human cell line RT-4 and human cell line U-251 MG.
Western Blot: SCAND1 Antibody [NBP2-56092] - Western blot analysis in human cell line RT-4 and human cell line U-251 MG.
Immunocytochemistry/ Immunofluorescence: SCAND1 Antibody [NBP2-56092] - Staining of human cell line PC-3 shows localization to nucleoplasm & cytosol.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ICC/IF
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

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SCAND1 Antibody Summary

Immunogen
This antibody was developed against a recombinant protein corresponding to the following amino acid sequence: PSSPNAAVPEAIPTPRAAASAALELPLGPAPVSVAPQAEAEARSTPGPAGSRLGPETFRQRF
Specificity
Specificity of human SCAND1 antibody verified on a Protein Array containing target protein plus 383 other non-specific proteins.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
SCAND1
Purity
Affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Western Blot 0.04-0.4 ug/ml
  • Immunocytochemistry/Immunofluorescence 0.25-2 ug/ml
Application Notes
Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.
Control
SCAND1 Knockout HeLa Cell Lysate
Control Peptide
SCAND1 Recombinant Protein Antigen (NBP2-56092PEP)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Affinity purified

Alternate Names for SCAND1 Antibody

  • RAZ1
  • SCAN domain containing 1
  • SCAN domain-containing 1
  • SCAN-related protein RAZ1
  • SDP1SCAN domain-containing protein 1

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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MZF1 Antibody
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Immunocytochemistry/Immunofluorescence: MZF1 Antibody [NBP1-82605] - Staining of human cell line A-431 shows localization to nucleus. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: MZF1 Antibody [NBP1-82605] - Staining of human cerebral cortex shows strong nuclear positivity in glial cells.
Species: Hu
Applications: ICC/IF, IHC, IHC-P
ZNF202 Antibody
(1E9)
H00007753-M01
Immunohistochemistry-Paraffin: ZNF202 Antibody (1E9) [H00007753-M01] - Analysis of monoclonal antibody to ZNF202 on formalin-fixed paraffin-embedded human prostate. Antibody concentration 3 ug/ml.
Sandwich ELISA: ZNF202 Antibody (1E9) [H00007753-M01] - Detection limit for recombinant GST tagged ZNF202 is approximately 0.1ng/ml as a capture antibody.
Species: Hu
Applications: WB, ELISA, IHC, IHC-P, S-ELISA
MEK1 Antibody
(SZ22-01)
NBP2-67358
Western Blot: MEK1 Antibody (SZ22-01) [NBP2-67358] - Whole cell lysates (30 ug per lane) from three different hematological cancer cell lines. Antibody at 1:1000. Western blot image submitted by a verified customer review.
Immunocytochemistry/Immunofluorescence: MEK1 Antibody (SZ22-01) [NBP2-67358] - Staining MEK1 in NIH/3T3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X-100/PBS.
Immunohistochemistry-Paraffin: MEK1 Antibody (SZ22-01) [NBP2-67358] - Analysis of paraffin-embedded mouse uterus tissue using anti-MEK1 antibody. Counter stained with hematoxylin.
(1 Review)
Species: Hu, Mu, Rt, Bv, Ca
Applications: WB, Flow, ICC/IF, IHC, IHC-P, IP
ZSCAN21/ZFP38
Antibody (OTI2E5)
NBP2-45443
Western Blot: ZSCAN21/ZFP38 Antibody (OTI2E5) [NBP2-45443] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ZSCAN21.
Immunohistochemistry: ZSCAN21/ZFP38 Antibody (OTI2E5) [NBP2-45443] - Analysis of Carcinoma of Human thyroid tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)
Western Blot: ZSCAN21/ZFP38 Antibody (OTI2E5) [NBP2-45443] - ZSCAN21/ZFP38 Antibody (2E5) [NBP2-45443] - Analysis of extracts (35ug) from 9 different cell lines (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).
Species: Hu, Mu, Ca, Pm
Applications: WB, IHC, IHC-P, IF
SHP-2/PTPN11
Antibody (6D9)
NBP2-37454
Western Blot: SHP-2/PTPN11 Antibody (6D9) [NBP2-37454] - Analysis using PTPN11 mAb against human PTPN11 recombinant protein. (Expected MW is 33.4 kDa)
Immunocytochemistry/Immunofluorescence: SHP-2/PTPN11 Antibody (6D9) [NBP2-37454] - Analysis of HeLa cells using PTPN11 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye.
Immunohistochemistry-Paraffin: SHP-2/PTPN11 Antibody (6D9) [NBP2-37454] - Analysis of breast cancer tissues using PTPN11 mouse mAb with DAB staining.
Species: Hu
Applications: WB, ELISA, Flow, ICC/IF, IHC, IHC-P, CyTOF-ready
ZNF24 Antibody
NBP1-82866
Western Blot: ZNF24 Antibody [NBP1-82866] - Analysis in human cell line SCLC-21H.
Immunocytochemistry/Immunofluorescence: ZNF24 Antibody [NBP1-82866] - Immunofluorescent staining of human cell line U-251 MG shows localization to nucleus.
Immunohistochemistry-Paraffin: ZNF24 Antibody [NBP1-82866] - Staining of human lymph node shows strong cytoplasmic and nuclear positivity in subsets of cells.
(1 Review)
(1 Publication)
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P, KD
ACE/CD143 Antibody
[Unconjugated]
AF1513
ACE/CD143 was detected in perfusion fixed frozen sections of mouse kidney using 15 µg/mL Goat Anti-Mouse ACE/CD143 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1513) overnight at 4 °C. Tissue was stained (red). View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections.
Western blot shows lysates of mouse lung tissue. PVDF membrane was probed with 0.05 µg/mL of Goat Anti-Mouse ACE/CD143 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1513) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for ACE/CD143 at approximately 180 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Simple Western lane view shows lysates of mouse lung tissue, loaded at 0.2 mg/mL. A specific band was detected for ACE/CD143 at approximately 218 kDa (as indicated) using 10 µg/mL of Goat Anti-Mouse ACE/CD143 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1513) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 66-440 kDa separation system.
(1 Review)
(3 Publications)
Species: Mu
Applications: WB, Simple Western, Flow, IHC, IP, CyTOF-ready
Lipoprotein
Lipase/LPL Antibody [ ...
AF7197
Western blot shows lysates of SH‑SY5Y human neuroblastoma cell line and NMuMG mouse mammary gland epithelial cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human Lipoprotein Lipase/LPL Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7197) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for Lipoprotein Lipase/LPL at approximately 56 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Lipoprotein Lipase/LPL was detected in immersion fixed paraffin-embedded sections of human heart using Goat Anti-Human/Mouse Lipoprotein Lipase/LPL Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7197) at 3 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Goat IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC004). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cardiomyocytes. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.
Lipoprotein Lipase/LPL was detected in immersion fixed SH‑SY5Y human neuroblastoma cell line using Goat Anti-Human Lipoprotein Lipase/LPL Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7197) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red, upper panel; Catalog # NL001) and counterstained with DAPI (blue, lower panel). Specific staining was localized to cell surfaces and cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips. This application has not been tested in mouse samples.
(1 Publication)
Species: Hu, Mu
Applications: WB, IHC, ICC
ZNF187 Antibody
NBP2-20970
Western Blot: ZNF187 Antibody [NBP2-20970] - Sample (30 ug of whole cell lysate) A: 293T 10% SDS PAGE gel, diluted at 1:5000.
Immunohistochemistry-Paraffin: ZNF187 Antibody [NBP2-20970] - Immunohistochemical analysis of paraffin-embedded TOV-21G xenograft, using antibody at 1:500 dilution.
Species: Hu
Applications: WB, IHC, IHC-P
CETP Antibody
(ATM192)
NB300-558
Western Blot: CETP Antibody (ATM192) [NB300-558] - Analysis of human liver extract.
Immunocytochemistry/Immunofluorescence: CETP Antibody (ATM192) [NB300-558] - Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with a CETP monoclonal antibody at a dilution of 1:20 overnight at 4 C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody and nuclei with DAPI (blue) is shown.
(1 Publication)
Species: Hu, Bv, Rb
Applications: WB, ELISA, ICC/IF, IP
ZNF174 Antibody
(2D7-E9)
H00007727-M01
Western Blot: ZNF174 Antibody (3E4) [H00007727-M01] - ZNF174 monoclonal antibody (M01), clone 2D7-E9 Analysis of ZNF174 expression in SW-13.
Immunocytochemistry/Immunofluorescence: ZNF174 Antibody (3E4) [H00007727-M01] - Analysis of monoclonal antibody to ZNF174 on HeLa cell. Antibody concentration 10 ug/ml.
Western Blot: ZNF174 Antibody (3E4) [H00007727-M01] - Analysis of ZNF174 over-expressed 293 cell line, cotransfected with ZNF174 Validated Chimera RNAi ( Cat # H00007727-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with ZNF174 monoclonal antibody (M01), clone 2D7-E9 (Cat # H00007727-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.
Species: Hu
Applications: WB, ELISA, ICC/IF, RNAi, S-ELISA
SNX3 Antibody
NBP2-98615
Western Blot: SNX3 Antibody [NBP2-98615] - Anti-SNX3 rabbit polyclonal antibody at 1:500 dilution. Lane A: HL-60 Whole Cell Lysate Lane B: THP1 Whole Cell Lysate Lane C: U-251M Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 19 kDa. Observed band size: 19 kDa
Immunocytochemistry/Immunofluorescence: SNX3 Antibody [NBP2-98615] - Immunofluorescence staining of SNX3 in A431 cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS, blocked with 10% serum, and incubated with rabbit anti-Human SNX3 polyclonal antibody (dilution ratio 1:100) at 4C overnight. Then cells were stained with the Alexa Fluor(R)488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue).Positive staining was localized to Cytoplasm.
Immunohistochemistry-Paraffin: SNX3 Antibody [NBP2-98615] - Immunochemical staining of human SNX3 in human kidney with rabbit polyclonal antibody at 1:100 dilution, formalin-fixed paraffin embedded sections.
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
Pepsinogen A
Antibody (961902)
MAB9350
Western Blot: Pepsinogen A Antibody (961902) [MAB9350] - Analysis of human stomach tissue lysate with 2 ug/ml of hPGA-4 Monoclonal Antibody followed by HAF005. A specific band was detected for hPGA-4 at approximately45 kDA (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Immunohistochemistry: Pepsinogen A Antibody (961902) [MAB9350] - Pepsinogen A was detected in immersion fixed paraffin-embedded sections of human stomach using Pepsinogen A Monoclonal Antibody at 1.7 ug/ml. Tissue was stained using Rat IgG VisUCyte HRP Polymer Antibody (brown) and counterstained with hematoxylin (blue). Specific staining was localized to the cytoplasm in epithelial cells.
(1 Review)
Species: Hu
Applications: WB, IHC, IHC-P
S5a/Angiocidin
Antibody
NBP2-37888
Western Blot: S5a/Angiocidin Antibody [NBP2-37888] - Analysis in control (vector only transfected HEK293T lysate) and PSMD4 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).
Immunocytochemistry/Immunofluorescence: S5a/Angiocidin Antibody [NBP2-37888] - Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm & cytosol.
Immunohistochemistry-Paraffin: S5a/Angiocidin Antibody [NBP2-37888] - Staining of human liver.
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
HO-1/HMOX1/HSP32
Antibody (HO-1-1)
NBP1-97507
Western Blot: HO-1/HMOX1/HSP32 Antibody (HO-1-1) [NBP1-97507] - Lane 1: MW marker; Lane 2: HO-1 (rat), (recombinant); Lane 3: HO-1 (human), (recombinant); Lane 4: HO-2 (human), (recombinant); Lane 5: MDBK cell lysate; Lane 6: Dog liver microsome; Lane 7: Mouse liver microsome.
Immunohistochemistry-Paraffin: HO-1/HMOX1/HSP32 Antibody (HO-1-1) [NBP1-97507] - Analysis of human spleen tissue stained with HO-1, mAb (HO-1-1) at 10ug/ml.
Flow Cytometry: HO-1/HMOX1/HSP32 Antibody (HO-1-1) [NBP1-97507] - Analysis of 10^6 Jurkat cells stained using HO-1 monoclonal antibody (HO-1-1), (Prod. No. ADI-OSA-110), at a concentration of 50 ug/mL
(1 Review)
(13 Publications)
Species: Hu, Mu, Rt, Bv, Ca
Applications: WB, Simple Western, Flow, ICC/IF, IHC, IHC-P
Plastin L Antibody
NBP2-19860
Western Blot: Plastin L Antibody [NBP2-19860] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with L-Plastin antibody diluted at 1:10000. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Immunocytochemistry/Immunofluorescence: Plastin L Antibody [NBP2-19860] - Plastin L antibody detects Plastin L protein by immunofluorescent analysis. Sample: A549 cells were fixed in ice-cold MeOH for 5 min. Green: Plastin Lstained by Plastin L antibody diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar= 10 um.
Immunohistochemistry-Paraffin: Plastin L Antibody [NBP2-19860] - Paraffin-embedded mouse thymus gland. Plastin L antibody diluted at 1:500.
Species: Hu, Mu
Applications: WB, ICC/IF, IHC, IHC-P
PGA5 [Unconjugated]
8457-AS
PGA5 [Unconjugated]
Species: Hu
SCAND1 Antibody
NBP2-56092
SCAND1 Antibody
Species: Hu
Applications: WB, ICC/IF

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Control Lysate(s)

SCAND1 Knockout HeLa Cell Lysate

Secondary Antibodies

 

Isotype Controls

Other Available Formats

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Bioinformatics

Gene Symbol SCAND1