ZNRF2 Antibody - BSA Free

Western Blot: ZNRF2 Antibody [NBP1-28715] - Samples: Whole cell lysate from HeLa (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded) and 293T (T; 50 ug) cells. Antibodies: Affinity purified rabbit anti-ZNRF2 antibody NBP1-28715 used for WB at 0.4 ug/ml (A) and 1 ug/ml (B) and used for IP at 10 ug/mg lysate. ZNRF2 was also immunoprecipitated by rabbit anti-ZNRF2 antibody NBP1-28697, which recognizes an upstream epitope. Detection: Chemiluminescence with exposure times of 3 minutes (A) and 30 seconds (B).

• Reactivity: Hu, Mu, Ze
• Applications: WB, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1.0 mg/ml

ZNRF2 Antibody - BSA Free Summary

• Immunogen: The immunogen recognized by this antibody maps to a region between residue 192 and 242 of human zinc and ring finger 2 using the numbering given in entry NP_667339.1 (GeneID 223082).
• Predicted Species: Mouse (100%), Zebrafish (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: ZNRF2
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunoprecipitation 10 ug/mg lysate
  • Western Blot 1:1000-1:5000

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: Tris-Citrate/Phosphate (pH 7.0 - 8.0)
• Preservative: 0.09% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for ZNRF2 Antibody - BSA Free

• EC 6.3.2.-
• Protein Ells2
• RING finger protein 202
• RNF202E3 ubiquitin-protein ligase ZNRF2
• zinc and ring finger 2
• zinc finger/RING finger 2
• Zinc/RING finger protein 2

Background

Zinc- and RING- finger 2 (ZNRF2) is suggested to play a role in the establisment and maintenance of neuronal transmission and plasticity via its ubiquitin ligase activity. E3 ubiquitin ligases accept ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfer the ubiquitin to targeted substrates.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for ZNRF2 Antibody (NBP1-28715)(1)

Publication using NBP1-28715

• Shannon Nicolson, Jantina A. Manning, Yoon Lim, Xin Jiang, Erica Kolze, Sonia Dayan, Ruchi Umargamwala, Tianqi Xu, Jarrod J. Sandow, Andrew I. Webb, Sharad Kumar, Donna Denton The Drosophila ZNRF1/2 homologue, detour, interacts with HOPS complex and regulates autophagy Communications Biology 2024-02-15 [PMID: 38360932]

Bioinformatics

• Gene Symbol: ZNRF2
• Entrez:
  • 223082(Human)
• Uniprot:
  • NP_667339.1(Human)