Recombinant Human ZNHIT3 His Protein Summary
| Description |
A denatured recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 1-155 of Human ZNHIT3 Source: E.coli Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MGSMASLKCS TVVCVICLEK PKYRCPACRV PYCSVVCFRK HKEQCNPETR PVEKKIRSAL PTKTVKPVEN KDDDDSIADF LNSDEEEDRV SLQNLKNLGE SATLRSLLLN PHLRQLMVNL DQGEDKAKLM RAYMQEPLFV EFADCCLGIV EPSQNEES |
| Source |
E. coli |
| Protein/Peptide Type |
Recombinant Protein |
| Gene |
ZNHIT3 |
| Purity |
>85%, by SDS-PAGE |
Applications/Dilutions
| Dilutions |
|
| Application Notes |
Denatured protein is most likely not the best option for functional studies. It is better suited for Western Blot (WB) or imaging assays. |
| Theoretical MW |
20 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
Packaging, Storage & Formulations
| Storage |
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer |
20 mM Tris-HCl buffer (pH 8.0), 10% glycerol, 0.4M UREA |
| Preservative |
No Preservative |
| Concentration |
1 mg/ml |
| Purity |
>85%, by SDS-PAGE |
Alternate Names for Recombinant Human ZNHIT3 His Protein
Background
ZNHIT3 contains 1 HIT-type zinc finger. Thyroid receptor interacting proteins (TRIPs) specifically interact with the ligand binding domain of the thyroid receptor (TR). This protein requires the presence of thyroid hormone for its interaction. Recombinant human ZNHIT3 protein, fused to His-tag at N-terminus, was expressed in E.coli .
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are
guaranteed for 3 months from date of receipt.
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