PLC-delta 3 Antibody

Immunohistochemistry-Paraffin: PLC-delta 3 Antibody [NBP3-20228] - Negative Control showing staining of paraffin embedded Human Testis, with no primary antibody.

Immunohistochemistry-Paraffin: PLC-delta 3 Antibody [NBP3-20228] - (6ug/ml) staining of paraffin embedded Human Testis. Heat induced antigen retrieval with citrate buffer pH 6, HRP-staining.

Immunohistochemistry-Paraffin: PLC-delta 3 Antibody [NBP3-20228] - Negative Control showing staining of paraffin embedded Human Kidney, with no primary antibody.

Immunohistochemistry-Paraffin: PLC-delta 3 Antibody [NBP3-20228] - (6ug/ml) staining of paraffin embedded Human Kidney. Heat induced antigen retrieval with citrate buffer pH 6, HRP-staining.

Western Blot: PLC-delta 3 Antibody [NBP3-20228] - (2ug/ml) staining of A549 (A) and Caco-2 (B) cell lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.

• Reactivity: Hu, Mu, Rt, Po, Bv, Ca
• Applications: WB, ELISA, IHC
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

PLC-delta 3 Antibody Summary

• Description: Novus Biologicals Goat PLC-delta 3 Antibody (NBP3-20228) is a polyclonal antibody validated for use in IHC, WB and ELISA. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Peptide with sequence C-HQYSGEDRVLSAPE, from the internal region of the protein sequence according to NP_588614.1.
• Epitope: aa261-274
• Predicted Species: Mouse (100%), Rat (100%), Porcine (100%), Bovine (100%), Canine (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: PLCD3
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 6 ug/ml
  • Peptide ELISA Detection limit 1:32000
  • Western Blot 1-3 ug/ml
• Application Notes: Western blot: Approx 85kDa band observed in lysates of cell limes A549 and Caco-2, and in preliminary testing of cell lines HeLa, HEK293 and U251 lysate (calculated MW of 89.3kDa according to NP_588614.1). Primary incubation 1 hour at room temperature.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for PLC-delta 3 Antibody

• EC 3.1.4.11
• KIAA1964
• MGC71172
• Phosphoinositide phospholipase C-delta-3
• phospholipase C, delta 3,1-phosphatidylinositol-4,5-bisphosphate phosphodiesterase delta-3
• Phospholipase C-delta-3
• PLC delta3
• PLCD3
• PLCdelta 3
• PLC-delta 3
• PLC-delta-3

Background

PLCD3, also referred to as Phospholipase C-delta-3, is 789 amino acids in length and approximately 89kDa. PLCD3 is a member of the Phospholipase C family and functions to hydrolyze PIP2 to produce the second messangers DAG and IP3. Current research on PLCD3 is being conducted in relation to several diseases and disorders including breast cancer, glioblastoma, Huntington's disease and neurological diseases. PLCD3 has also been shown to have interactions with ITPR1, ITPR3, PIK3C3, DGKE and SKIL in pathways such as the CREB pathway, sweet taste signaling and Inositol phosphate metabolism.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: PLCD3