Home » ZNF521 » ZNF521 Antibodies » ZNF521 Antibody [DyLight 650]

ZNF521 Antibody [DyLight 650]

 Submit a Review

Images

 
There are currently no images for ZNF521 Antibody (NBP1-91270C).

Every product we sell is backed by Novus' 100% Guarantee. If you have used this product, please submit your images and reviews to earn reward points.

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications ICC/IF, IHC, IHC-P, WB (-)
Clonality
Polyclonal
Host
Rabbit
Conjugate
DyLight 650

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
NBP1-91270AF350
Alexa Fluor 405
NBP1-91270AF405
Alexa Fluor 488
NBP1-91270AF488
Alexa Fluor 532
NBP1-91270AF532
Alexa Fluor 594
NBP1-91270AF594
Alexa Fluor 647
NBP1-91270AF647
Alexa Fluor 700
NBP1-91270AF700
Alexa Fluor 750
NBP1-91270AF750
Biotin
NBP1-91270B
DyLight 350
NBP1-91270UV
DyLight 405
NBP1-91270V
DyLight 488
NBP1-91270G
DyLight 550
NBP1-91270R
DyLight 594
NBP1-91270DL594
DyLight 680
NBP1-91270FR
DyLight 755
NBP1-91270IR
FITC
NBP1-91270F
HRP
NBP1-91270H
Janelia Fluor 549
NBP1-91270JF549
Janelia Fluor 646
NBP1-91270JF646
Unconjugated
NBP1-91270
100% Guarantee on every product

ZNF521 Antibody [DyLight 650] Summary

Immunogen
A synthetic peptide made to an internal portion of the human ZNF521 protein (between residues 200-250) [UniProt Q96K83]
Localization
Nucleus and cytoplasm.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
ZNF521
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Immunocytochemistry/Immunofluorescence 1:40
  • Immunohistochemistry 1:400
  • Immunohistochemistry-Paraffin 1:400
Application Notes
This ZNF521 antibody is useful for Immunocytochemistry/Immunofluorescence and IHC-paraffin embedded sections. In ICC/IF nuclear staining was observed in U2OS cells. In IHC-P, staining was observed in the cytoplasm and nucleus of mouse epidermis tissue. Prior to immunostaining paraffin tissues, antigen retrieval with sodium citrate buffer (pH 6.0) is recommended. This antibody is not recommended for Western Blot analysis.

Reactivity Notes

Human and mouse.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
50mM Sodium Borate
Preservative
0.05% Sodium Azide
Purity
Immunogen affinity purified

Notes

Dylight (R) is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries. This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.

Alternate Names for ZNF521 Antibody [DyLight 650]

  • EHZF
  • Evi3
  • LIP3
  • ZFP521
  • zinc finger protein 263
  • Zinc finger protein FPM315
  • Zinc finger protein with KRAB and SCAN domains 12

Background

Zinc finger protein 521 (ZNF521; EHZF) is an 1131 amino-acid long transcription factor that includes 30 zinc fingers which are scattered along its sequence. Zfp521 appears to play a role in development, bone formation, erythroid cell differentiation, stem cell regulation and oncogenesis. Zfp521 is expressed in developing bones, where it binds to Runx2, thusly repressing the transcriptional activity of Runx2. This balance between Zfp521 and Runx2 may play a role in the regulation of osteoblast differentiation and bone formation (PMID: 20392215). Zinc finger protein 521 has also been shown to be expressed in hematopoietic stem and progenitor cells but is down-regulated as they go through the process of differentiation (PMID:19342626). Furthermore, ZNF521 was shown to inhibit the activity of GATA-1 which is a crucial regulator of erythroid differentiation by directly binding to GATA-1 (PMID: 19049973). Expression of ZNF521 has been detected in most acute myelogenous leukaemias, as well as in medulloblastomas and other brain tumors (PMID: 17543573).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

EBF-1 Antibody
(1G8)
H00001879-M02
Western Blot: EBF-1 Antibody (1G8) [H00001879-M02] - Analysis of EBF1 expression in transfected 293T cell line by EBF1 monoclonal antibody (M02), clone 1G8.Lane 1: EBF1 transfected lysate(64 KDa).Lane 2: Non-transfected lysate.
Western Blot: EBF-1 Antibody (1G8) [H00001879-M02] - Analysis of EBF1 over-expressed 293 cell line, cotransfected with EBF1 Validated Chimera RNAi ( Cat # H00001879-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with EBF1 monoclonal antibody (M02), clone 1G8 (Cat # H00001879-M02 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.
Sandwich ELISA: EBF-1 Antibody (1G8) [H00001879-M02] - Detection limit for recombinant GST tagged EBF1 is approximately 0.1ng/ml as a capture antibody.
(2 Publications)
Species: Hu, Mu
Applications: WB, ChIP, ELISA, IP, RNAi, S-ELISA
CENPJ Antibody
(1A5)
H00055835-M02
Western Blot: CENPJ Antibody (1A5) [H00055835-M02] - Analysis of CENPJ expression in transfected 293T cell line by CENPJ monoclonal antibody (M02), clone 1A5.Lane 1: CENPJ transfected lysate(43.2 KDa).Lane 2: Non-transfected lysate.
Immunoprecipitation: CENPJ Antibody (1A5) [H00055835-M02] - Analysis of CENPJ transfected lysate using anti-CENPJ monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with CENPJ MaxPab rabbit polyclonal antibody.
Sandwich ELISA: CENPJ Antibody (1A5) [H00055835-M02] - Detection limit for recombinant GST tagged CENPJ is 0.1 ng/ml as a capture antibody.
Species: Hu
Applications: WB, ELISA, IP, S-ELISA
Liprin alpha 1
Antibody
21720002
Western Blot: Liprin alpha 1 Antibody [21720002] - Samples: Lane 1, Marker [kDa]: 250, 130, 95, 72, 55, 36, 28, 17, 11 Lane 2, RT-4 Lane 3, U-251MG sp Lane 4, Human Plasma Lane 5, Liver Lane 6, Tonsil, Target weight [kDa]: 136, 134 (splice variants) Validation score: 2 Validation description: Supportive - Band of predicted size in kDa (+/-20%) with additional bands present.
Immunohistochemistry: Liprin alpha 1 Antibody [21720002] - Most of the normal tissues displayed moderate cytoplasmic positivity which was occasionally accompanied with nuclear staining. Strong cytoplasmic staining was found in gastro-intestinal tract, gall bladder and endocrine glands. Malignant tissues generally exhibited moderate cytoplasmic immunoreactivity. A few cases of colo-rectal, endometrial, gastric and pancreatic cancers revealed strong staining while many skin cancers were weakly stained. Image and statement courtesy of the Human Protein Atlas (HPA).
ELISA: Liprin alpha 1 Antibody [21720002]
(2 Publications)
Species: Hu
Applications: WB, ELISA, IHC, IHC-P
LIAS Antibody
NBP1-82845
Western Blot: LIAS Antibody [NBP1-82845] - Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells). Lane 2: NBT-II cell lysate (Rat Wistar bladder tumor cells).
Immunocytochemistry/Immunofluorescence: LIAS Antibody [NBP1-82845] - Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm & mitochondria. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: LIAS Antibody [NBP1-82845] - Staining of mouse lateral ventricle shows positivity in a subset of cells in choroid plexus and lateral ventricle wall.
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P
STK11IP Antibody
NBP1-28481
Western Blot: STK11IP Antibody [NBP1-28481] - (0.5ug/ml) staining of Human Ovary lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
(1 Publication)
Species: Hu
Applications: WB, PEP-ELISA
Annexin A2
Antibody [Unconjugate
AF3928
Western blot shows lysates of A431 human epithelial carcinoma cell line, NIH-3T3 mouse embryonic fibroblast cell line, and L6 rat myoblast cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human/Mouse/Rat Annexin A2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3928) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for Annexin A2 at approximately 39 kDa (as indicated). This experiment was conducted using Immunoblot Buffer Group 2.
Annexin A2 was detected in immersion fixed paraffin-embedded sections of normal human liver using Goat Anti-Human/Mouse/Rat Annexin A2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3928) at 3 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to plasma membranes of hepatocytes. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Simple Western lane view shows lysates of A431 human epithelial carcinoma cell line and NIH‑3T3 mouse embryonic fibroblast cell line, loaded at 0.2 mg/mL. A specific band was detected for Annexin A2 at approximately 45 kDa (as indicated) using 50 µg/mL of Goat Anti-Human/Mouse/Rat Annexin A2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3928) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12‑230 kDa separation system. Non-specific interaction with the 230 kDa Simple Western standard may be seen with this antibody.
(1 Review)
(2 Publications)
Species: Hu, Mu, Rt
Applications: WB, Simple Western, IHC, KO
MRPL17 Antibody
NBP2-13611
Western Blot: MRPL17 Antibody [NBP2-13611] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG sp. Lane 4: Human plasma (IgG/HSA depleted). Lane 5: Human liver tissue. Lane 6: Human tonsil tissue
Immunohistochemistry-Paraffin: MRPL17 Antibody [NBP2-13611] - Staining of human breast shows strong cytoplasmic positivity in granular pattern glandular cells.
Species: Hu
Applications: WB, IHC, IHC-P
ZNF423 Antibody
NBP2-76351
Immunocytochemistry/Immunofluorescence: ZNF423 Antibody [NBP2-76351] - Analysis of paraformaldehyde fixed A431 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL), showing nuclear staining. Actin filaments were stained with phalloidin (red) and the nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL).
Flow Cytometry: ZNF423 Antibody [NBP2-76351] - Analysis of paraformaldehyde fixed Kelly cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (1 ug/mL). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.
Species: Hu
Applications: Flow, ICC/IF, PEP-ELISA
PTHLH/PTHrP
Antibody
NBP1-59322
Western Blot: PTHLH/PTHrP Antibody [NBP1-59322] - HepG2 cell lysate, Antibody Titration: 2.0ug/ml
Immunohistochemistry-Paraffin: PTHLH/PTHrP Antibody [NBP1-59322] - Human Lung Alveolar cells (indicated with arrows), 4-8ug/ml.
Western Blot: PTHLH/PTHrP Antibody [NBP1-59322] - Primary, Antibody Dilution: 1 : 1000 Secondary Antibody: Goat anti-rabbit HRP Secondary, Antibody Dilution: 1 : 2000.
Species: Hu
Applications: WB, IHC, IHC-P
BMP-2
[Unconjugated]
355-BM
BMP-2 [Unconjugated]
(9 Reviews)
(153 Publications)
Species: Hu, Mu, Rt
Recombinant Human
OR5I1 GST (N-Term) Pr ...
H00010798-P01
12.5% SDS-PAGE Stained with Coomassie Blue.
Species: Hu
Human PTH ELISA
Kit (Colorimetric)
NBP2-60629
ELISA: Human PTH ELISA Kit (Colorimetric) [NBP2-60629]
(1 Publication)
Species: Hu
VTA1 Antibody
NBP1-86745
Western Blot: VTA1 Antibody [NBP1-86745] - Analysis using Anti-VTA1 antibody NBP1-86745 (A) shows similar pattern to independent antibody NBP2-58305 (B).
Immunohistochemistry-Paraffin: VTA1 Antibody [NBP1-86745] - Staining of human fallopian tube shows moderate cytoplasmic positivity in glandular cells.
Immunohistochemistry-Paraffin: VTA1 Antibody [NBP1-86745] - Staining of human stomach shows strong cytoplasmic positivity in parietal cells.
Species: Hu, Mu, Rt
Applications: WB, IHC, IHC-P

Osteoprotegerin/TNFRSF
DY805
(63 Publications)
Species: Hu
RUNX2/CBFA1
Antibody
NBP1-77461
Immunohistochemistry-Paraffin: RUNX2/CBFA1 Antibody [NBP1-77461] - Vascularization of mineralized cell-laden collagen and interaction with prostate cancer cells. HUVECs formed endothelial networks that were supported by SMA-expressing hMSCs (scale bar: 50m) and were also positive for CD31 (scale bar: 400m). The remainder of hMSCs expressed RUNX2 as a marker for osteogenic differentiation (scale bar: 50m). Rapid fabrication of vascularized and innervated cell-laden bone models with biomimetic intrafibrillar collagen mineralization. Nat Commun (2019)
Immunocytochemistry/Immunofluorescence: RUNX2/CBFA1 Antibody [NBP1-77461] - RUNX2 antibody was tested in HeLa cells with FITC (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and DyLight 550 (red).
Immunohistochemistry-Paraffin: RUNX2/CBFA1 Antibody [NBP1-77461] - Characterization of TLC cultured in chondrogenic pellets. Gene expression of TLC cultured four weeks in chondrogenic pellets or in cell monolayer in control medium: levels of expression of osteoblast markers (1), chondrocyte markers (2) and tenocyte markers (3) (N = 3) assessed by qPCR.* =0.05. Rotator Cuff Tenocytes Differentiate into Hypertrophic Chondrocyte-Like Cells to Produce Calcium Deposits in an Alkaline Phosphatase-Dependent Manner. J Clin Med (2019)
(9 Publications)
Species: Hu, Mu
Applications: WB, ICC/IF, IHC, IHC-P
GATA-1 Antibody
(4F5)
NBP1-47492
Western Blot: GATA-1 Antibody (4F5) [NBP1-47492] - Analysis using GATA1 mouse mAb against K562 (1) cell lysate.
Immunocytochemistry/Immunofluorescence: GATA-1 Antibody (4F5) [NBP1-47492] - Analysis of K562(left) cells using GATA1 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye.
Immunohistochemistry: GATA-1 Antibody (4F5) [NBP1-47492] - Staining of Fetal Baboon Liver. Antigen retrieval: 10mM sodium citrate, pH 6.0, Primary Ab: 1:500 dilution, Secondary Ab (biotinylated): 1:200, Streptavidin-AlexaFluor488: 1:500 (green), Quench autofluorescence with 1% Sudan Black in 70% methanol. Image from verified customer review.
(1 Review)
(2 Publications)
Species: Hu, Pm
Applications: WB, ChIP, ELISA, ICC/IF, IHC, IHC-P
Osteopontin/OPN
Antibody [Unconjugate
AF808
Osteopontin/OPN was detected in immersion fixed C2C12 mouse myoblast cell line (left panel, positive stain) and mouse splenocytes (right panel, negative stain) using Goat Anti-Mouse Osteopontin/OPN Antigen Affinity-purified Polyclonal Antibody (Catalog # AF808) at 5 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm and secreted molecule. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Recombinant Mouse Osteopontin/OPN (Catalog # 441-OP), immobilized onto a microplate, supports the adhesion of the HEK293 human embryonic kidney cell line in a dose-dependent manner (orange line). Adhesion elicited by Recombinant Mouse Osteopontin/OPN (2 µg/mL) is neutralized (green line) by increasing concentrations of Mouse Osteopontin/OPN Antigen Affinity-purified Polyclonal Antibody (Catalog # AF808). The ND50 is typically 1‑3 µg/mL.
Osteopontin/OPN was detected in perfusion fixed frozen sections of mouse thymus using Mouse Osteopontin/OPN Antigen Affinity-purified Polyclonal Antibody (Catalog # AF808) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.
(7 Reviews)
(81 Publications)
Species: Mu
Applications: WB, IHC, ELISA(Cap), ELISA(Det), ICC, Neut, ELISA(Sta)
ZNF521 Antibody
[DyLight 650]
NBP1-91270C
ZNF521 Antibody [DyLight 650]
Species: Hu, Mu
Applications: ICC/IF, IHC, IHC-P, WB (-)

Publications for ZNF521 Antibody (NBP1-91270C) (0)

There are no publications for ZNF521 Antibody (NBP1-91270C).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for ZNF521 Antibody (NBP1-91270C) (0)

There are no reviews for ZNF521 Antibody (NBP1-91270C). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

ICC/IF Video Protocol

FAQs for ZNF521 Antibody (NBP1-91270C) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 NBP1-91270AF350
Alexa Fluor 405 NBP1-91270AF405
Alexa Fluor 488 NBP1-91270AF488
Alexa Fluor 532 NBP1-91270AF532
Alexa Fluor 594 NBP1-91270AF594
Alexa Fluor 647 NBP1-91270AF647
Alexa Fluor 700 NBP1-91270AF700
Alexa Fluor 750 NBP1-91270AF750
Biotin NBP1-91270B
DyLight 350 NBP1-91270UV
DyLight 405 NBP1-91270V
DyLight 488 NBP1-91270G
DyLight 550 NBP1-91270R
DyLight 594 NBP1-91270DL594
DyLight 650 NBP1-91270C
DyLight 680 NBP1-91270FR
DyLight 755 NBP1-91270IR
FITC NBP1-91270F
HRP NBP1-91270H
Janelia Fluor 549 NBP1-91270JF549
Janelia Fluor 646 NBP1-91270JF646
Learn about Custom Antibody Labeling

Additional ZNF521 Products

Bioinformatics Tool for ZNF521 Antibody (NBP1-91270C)

Discover related pathways, diseases and genes to ZNF521 Antibody (NBP1-91270C). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for ZNF521 Antibody (NBP1-91270C)

Discover more about diseases related to ZNF521 Antibody (NBP1-91270C).
 

Pathways for ZNF521 Antibody (NBP1-91270C)

View related products by pathway.

Research Areas for ZNF521 Antibody (NBP1-91270C)

Find related products by research area.

Blogs on ZNF521

There are no specific blogs for ZNF521, but you can read our latest blog posts.
Coronavirus Brochure

Customers Who Bought This Also Bought

BMP-2 [Unconjugated]
355-BM-010

Contact Information

Learn the difference between western blot and simple western

Product PDFs

  SDS
  PDF Datasheet
  Request CofA

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our ZNF521 Antibody [DyLight 650] and receive a gift card or discount.


Bioinformatics

Gene Symbol ZNF521