ZNF521 Antibody [DyLight 650]

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Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications ICC/IF, IHC, IHC-P, WB (-)
Clonality
Polyclonal
Host
Rabbit
Conjugate
DyLight 650

ZNF521 Antibody [DyLight 650] Summary

Immunogen
A synthetic peptide made to an internal portion of the human ZNF521 protein (between residues 200-250) [UniProt Q96K83]
Localization
Nucleus and cytoplasm.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
ZNF521
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/Immunofluorescence 1:40
  • Immunohistochemistry 1:400
  • Immunohistochemistry-Paraffin 1:400
Application Notes
This ZNF521 antibody is useful for Immunocytochemistry/Immunofluorescence and IHC-paraffin embedded sections. In ICC/IF nuclear staining was observed in U2OS cells. In IHC-P, staining was observed in the cytoplasm and nucleus of mouse epidermis tissue. Prior to immunostaining paraffin tissues, antigen retrieval with sodium citrate buffer (pH 6.0) is recommended. This antibody is not recommended for Western Blot analysis.

Reactivity Notes

Human and mouse.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
50mM Sodium Borate
Preservative
0.05% Sodium Azide
Purity
Immunogen affinity purified

Notes

Dylight (R) is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries. This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.

Alternate Names for ZNF521 Antibody [DyLight 650]

  • EHZF
  • Evi3
  • LIP3
  • ZFP521
  • zinc finger protein 263
  • Zinc finger protein FPM315
  • Zinc finger protein with KRAB and SCAN domains 12

Background

Zinc finger protein 521 (ZNF521; EHZF) is an 1131 amino-acid long transcription factor that includes 30 zinc fingers which are scattered along its sequence. Zfp521 appears to play a role in development, bone formation, erythroid cell differentiation, stem cell regulation and oncogenesis. Zfp521 is expressed in developing bones, where it binds to Runx2, thusly repressing the transcriptional activity of Runx2. This balance between Zfp521 and Runx2 may play a role in the regulation of osteoblast differentiation and bone formation (PMID: 20392215). Zinc finger protein 521 has also been shown to be expressed in hematopoietic stem and progenitor cells but is down-regulated as they go through the process of differentiation (PMID:19342626). Furthermore, ZNF521 was shown to inhibit the activity of GATA-1 which is a crucial regulator of erythroid differentiation by directly binding to GATA-1 (PMID: 19049973). Expression of ZNF521 has been detected in most acute myelogenous leukaemias, as well as in medulloblastomas and other brain tumors (PMID: 17543573).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

ICC/IF Video Protocol

FAQs for ZNF521 Antibody (NBP1-91270C) (0)

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Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 NBP1-91270AF350
Alexa Fluor 405 NBP1-91270AF405
Alexa Fluor 488 NBP1-91270AF488
Alexa Fluor 532 NBP1-91270AF532
Alexa Fluor 594 NBP1-91270AF594
Alexa Fluor 647 NBP1-91270AF647
Alexa Fluor 700 NBP1-91270AF700
Alexa Fluor 750 NBP1-91270AF750
Biotin NBP1-91270B
DyLight 350 NBP1-91270UV
DyLight 405 NBP1-91270V
DyLight 488 NBP1-91270G
DyLight 550 NBP1-91270R
DyLight 594 NBP1-91270DL594
DyLight 650 NBP1-91270C
DyLight 680 NBP1-91270FR
DyLight 755 NBP1-91270IR
FITC NBP1-91270F
HRP NBP1-91270H
Janelia Fluor 549 NBP1-91270JF549
Janelia Fluor 646 NBP1-91270JF646

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Bioinformatics Tool for ZNF521 Antibody (NBP1-91270C)

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Diseases for ZNF521 Antibody (NBP1-91270C)

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Contact Information

Learn the difference between western blot and simple western

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Calculators

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Bioinformatics

Gene Symbol ZNF521