ZNF274 Antibody - BSA Free

Western Blot: ZNF274 Antibody [NBP1-80597] - Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11. Lane 2: Human cell line RT-4.

Immunohistochemistry-Paraffin: ZNF274 Antibody [NBP1-80597] - Staining of human liver shows strong cytoplasmic and nuclear positivity in hepatocytes.

• Reactivity: Hu
• Applications: WB, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

ZNF274 Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit ZNF274 Antibody - BSA Free (NBP1-80597) is a polyclonal antibody validated for use in IHC and WB. Anti-ZNF274 Antibody: Cited in 2 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: This antibody was developed against Recombinant Protein corresponding to amino acids: FGHLVSVGWETTLENKELAPNSDIPEEEPAPSLKVQESSRDCALSSTLEDTLQGGVQEVQDTVLKQMESAQEKDLPQKKHFDNRESQANSGALDTNQVSLQKIDNPESQANSGALDTNQVLLHKIPPRKRLRKRDSQVKSMK
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: ZNF274
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:50 - 1:200
  • Immunohistochemistry-Paraffin 1:50 - 1:200
  • Western Blot 0.04-0.4 ug/ml
• Application Notes: For IHC-Paraffin, HIER pH 6 retrieval is recommended. IF fixation permeabilization: PFA/Triton X-100.
• Control Peptide:

  ZNF274 Protein (NBP1-80597PEP)

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2) and 40% Glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Immunogen affinity purified

Alternate Names for ZNF274 Antibody - BSA Free

• GIOT2
• GIOT-2
• gonadotropin inducible transcription repressor-2
• Gonadotropin-inducible ovary transcription repressor 2
• gonadotropin-inducible transcription repressor 2
• KOX7DKFZp434F1811
• zinc finger protein 44 (KOX 7)
• zinc finger protein 44
• Zinc finger protein 55DKFZp686L21136
• Zinc finger protein 58ZNF504
• Zinc finger protein KOX7
• zinc finger protein ZnFP12
• ZNF
• ZNF55
• ZNF58

Background

ZNF274 encodes a zinc finger protein containing five C2H2-type zinc finger domains, one or two Kruppel-associated box A (KRAB A) domains, and a leucine-rich domain. The encoded protein has been suggested to be a transcriptional repressor. It localizes predominantly to the nucleolus. Alternatively spliced transcript variants encoding different isoforms exist. These variants utilize alternative polyadenylation signals.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for ZNF274 Antibody (NBP1-80597)(2)

Publications using NBP1-80597

• Wang N, Wang M, Jeevaratnam S et al. Opposing effects of apoE2 and apoE4 on microglial activation and lipid metabolism in response to demyelination Molecular neurodegeneration 2022-11-23 [PMID: 36419137] (IF/IHC, Mouse)
• Chen L, Zhang C, Ma W et al. METTL3-mediated m6A modification stabilizes TERRA and maintains telomere stability Nucleic acids research 2022-11-18 [PMID: 36399511] (WB, Human)
  
  Details:
  Dilution used in WB 1:1000

Bioinformatics

• Gene Symbol: ZNF274
• Entrez:
  • 10782(Human)