ZHX3 Antibody (PCRP-ZHX3-1D11)

Flow Cytometry: ZHX3 Antibody (PCRP-ZHX3-1D11) [NBP3-13888] - Flow cytometric analysis of PFA-fixed HeLa cells. ZHX3 antibody (PCRP-ZHX3-1D11) followed by goat anti-mouse IgG-CF488 (blue); isotype control (red).

Protein Array: ZHX3 Antibody (PCRP-ZHX3-1D11) [NBP3-13888] - Analysis of Protein Array containing more than 19,000 full-length human proteins using ZHX3 Antibody (PCRP-ZHX3-1D11).

• Reactivity: Hu
• Applications: WB, ELISA, Flow, IP, MA
• Clone: PCRP-ZHX3-1D11
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: 0.2 mg/ml

ZHX3 Antibody (PCRP-ZHX3-1D11) Summary

• Description: 200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP3-14225)
  
  Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80 C.
• Immunogen: Recombinant full-length human ZHX3 protein (Uniprot: Q9H4I2)
• Localization: Nucleus
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: ZHX3
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Flow Cytometry 1-2 ug/million cells
  • Immunoprecipitation 1-2 ug/100-500 ug protein
  • Protein Array
  • Western Blot 1-2 ug/ml
• Application Notes: ELISA: For coating, order antibody without BSA
  
  Immunoprecipitation: 1-2ug per 100-500ug of total protein (1ml of cell lysate)
• Theoretical MW: 105 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C.
• Buffer: 10 mM PBS with 0.05% BSA
• Preservative: 0.05% Sodium Azide
• Concentration: 0.2 mg/ml
• Purity: Protein A or G purified

Alternate Names for ZHX3 Antibody (PCRP-ZHX3-1D11)

• FLJ93221
• SP38
• zona pellucida binding protein
• zona pellucida-binding protein 1
• ZPBP1zona pellucida binding protein 1

Background

ZHX3 encodes a member of the zinc fingers and homeoboxes (ZHX) gene family. The encoded protein contains two C2H2-type zinc fingers and five homeodomains and forms a dimer with itself or with zinc fingers and homeoboxes family member 1. In the nucleus, the dimerized protein interacts with the A subunit of the ubiquitous transcription factor nuclear factor-Y and may function as a transcriptional repressor.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: ZHX3
• Uniprot:
  • Q9H4I2(Human)