ZHX1 Antibody - BSA Free

Western Blot: ZHX1 Antibody [NB600-244] - Whole cell lysate (15 ug) from HeLa, HEK293T, Jurkat, mouse TCMK-1, and mouse NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbitanti-ZHX1 antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

Immunohistochemistry-Paraffin: ZHX1 Antibody [NB600-244] - Section of human lung carcinoma. Antibody: Affinity purified rabbit anti- ZHX1 used at a dilution of 1:1,000 (1ug/ml). Detection: DAB

Immunoprecipitation: ZHX1 Antibody [NB600-244] - Detection of human ZHX1 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-ZHX1 antibody NB600-244 used for IP at 3 ug per reaction. ZHX1 was also immunoprecipitated by another rabbit anti-ZHX1 antibody. For blotting immunoprecipitated ZHX1, NB600-244 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 75 seconds.

• Reactivity: Hu, Mu, Pm
• Applications: WB, IHC, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1.0 mg/ml

ZHX1 Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit ZHX1 Antibody - BSA Free (NB600-244) is a polyclonal antibody validated for use in IHC, WB and IP. Anti-ZHX1 Antibody: Cited in 3 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: The immunogen this antibody was made to, maps to a region between residues 1-50 of human zinc fingers and homeoboxes 1 using the TrEMBL entry Q9UKY1 (GeneID 11244).
• Predicted Species: Primate (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: ZHX1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:500 - 1:2000
  • Immunohistochemistry-Paraffin 1:500 - 1:2000
  • Immunoprecipitation 2-5 ug/mg lysate
  • Western Blot 1:2000-1:10000
• Application Notes: Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.
• Control:

  HeLa Nuclear Cell Lysate

Reactivity Notes

Chimpanzee (100%).

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: Tris-Citrate/Phosphate (pH 7.0 - 8.0)
• Preservative: 0.09% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for ZHX1 Antibody - BSA Free

• zona pellucida B protein
• zona pellucida glycoprotein 4ZBP
• Zona pellucida protein B
• zona pellucida sperm-binding protein 4
• Zp-4
• ZPBZP1

Background

Zinc-fingers and homeoboxes 1 (ZHX1) is a protein which interacts with the activation domain of the A subunit of nuclear factor-Y. It is a transcriptional repressor protein.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for ZHX1 Antibody (NB600-244)(3)

Publications using NB600-244

• Chen Y, Wu Y, Li J et al. Cooperative regulation of Zhx1 and hnRNPA1 drives the cardiac progenitor-specific transcriptional activation during cardiomyocyte differentiation Cell death discovery 2023-07-14 [PMID: 37452012] (WB, IP, Mouse)
• Clement LC, Liu G, Perez-Torres I et al. Early changes in gene expression that influence the course of primary glomerular disease. Kidney Int 2007-04-25 [PMID: 17457373]
• Liu G, Clement LC, Kanwar YS et al. ZHX proteins regulate podocyte gene expression during the development of nephrotic syndrome. J Biol Chem 2006-12-22 [PMID: 17056598]

Bioinformatics

• Gene Symbol: ZHX1
• Entrez:
  • 11244(Human)
• Uniprot:
  • Q9UKY1(Human)