ZFP90 Antibody

Western blot shows lysates of IMR-32 human neuroblastoma cell line and Bowes human melanoma cell line. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human ZFP90 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7870) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for ZFP90 at approximately 72 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

• Reactivity: Hu
• Applications: WB
• Clonality: Polyclonal
• Host: Sheep
• Conjugate: Unconjugated
• Concentration: LYOPH

ZFP90 Antibody Summary

• Immunogen: E. coli-derived recombinant human ZFP90
  Lys94-Lys207
  Accession # Q8TF47
• Specificity: Detects human ZFP90 in direct ELISAs and Western blots. In direct ELISAs, less than 2% cross-reactivity with recombinant mouse ZFP90 is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Sheep
• Gene: ZFP90
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Sterile PBS to a final concentration of 0.2 mg/mL.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for ZFP90 Antibody

• FoxP3-interacting Protein
• KIAA1954
• NK10
• ZFP90
• zfp-90
• zinc finger protein 476
• zinc finger protein 756
• zinc finger protein 90 homolog (mouse)
• zinc finger protein 90 homolog
• ZNF756

Background

ZFP90 (Zinc Finger Protein 90; also ZFP756 and NK10) is a nuclear 66-72 kDa member of the ZNF505 clade, ZNF91 subfamily, kruppel C2H2-type zinc finger family of proteins. It is widely expressed, and found in cell types such as hepatocytes and cardiac myocytes. Functionally, ZFP90 acts as a transcriptional repressor by interacting with KAP-1/TRIM28/TIF1 beta . This interaction influences histone modifications and heterochromatin protein 1 deposition on DNA. Conversely, it is also known to bind to NRSF, thereby promoting NRSF sensitive gene expression. Human ZFP90 is 636 amino acids (aa) in length. It contains one KRAB domain (aa 14-85) followed by thirteen C2H2-type Zn-finger domains (aa 211-631). Over aa 94-207, human and mouse ZFP90 share 54% aa sequence identity.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: ZFP90
• Uniprot:
  • Q8TF47(Human)