Immunocytochemistry/ Immunofluorescence: ZEB1 Antibody (4C4) [H00006935-M01] - U-2 OS cells were stained with ZEB1-FITC labeled monoclonal antibody (Green). The cell nucleus were counterstained with DAPI (Blue).
Immunocytochemistry/ Immunofluorescence: ZEB1 Antibody (4C4) [H00006935-M01] - Analysis of monoclonal antibody to ZEB1 on HeLa cell . Antibody concentration 10 ug/ml.
Immunocytochemistry/ Immunofluorescence: ZEB1 Antibody (4C4) [H00006935-M01] - Analysis of monoclonal antibody to ZEB1 on U-2 OS cell. Antibody concentration 10 ug/ml.
ELISA: ZEB1 Antibody (4C4) [H00006935-M01] - Detection limit for recombinant GST tagged ZEB1 is 3 ng/ml as a capture antibody.
Quality control test: Antibody Reactive Against Recombinant Protein.
ZEB1 (NP_110378, 801 a.a. ~ 900 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. NIAIPTVTAQLPTIVAIADQNSVPCLRALAANKQTILIPQVAYTYSTTVSPAVQEPPLKVIQPNGNQDERQDTSSEGVSNVEDQNDSDSTPPKKKMRKTE
ZEB1 encodes a zinc finger transcription factor that represses T-lymphocyte-specific IL2 gene (MIM 147680) expression by binding to a negative regulatory domain 100 nucleotides 5-prime of the IL2 transcription start site (Williams et al., 1991 [PubMed 1840704]).[supplied by OMIM]
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
FAQs for ZEB1 Antibody (H00006935-M01). (Showing 1 - 1 of 1 FAQs).
In looking at the Zeb1 antibodies on your website, the molecular weights of Zeb1 detected by several antibodies are different. Why is this?
ZEB1 is a rather large protein and undergoes a lot of post translational modifications such as phosphorylation and glycosylation (please see: UniProt P37275). The reason band patterns are different in the images may be due to sample type and how highly modified the ZEB1 protein is that the antibody is detecting. The more post translational modifications the higher you will detect the protein.
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