ZCCHC8 Antibody - BSA Free

Western Blot: ZCCHC8 Antibody [NB100-94995] - Analysis of ZCCHC8 in HeLa whole cell lsyate using anti-ZCCHC8 antibody. Lane1: Control siRNA , Lane 2: HeLa cells treated with ZCCHC8 siRNA . Image from verified customer review.

Immunohistochemistry-Paraffin: ZCCHC8 Antibody [NB100-94995] - Sections of human breast carcinoma. Antibody: Affinity purified rabbit anti-used at a dilution of 1:5,000 (0.2ug/ml) and 1:2,000 (0.5ug/ml). Detection: DAB and Red-fluorescent Goat anti-Rabbit IgG-heavy and light chain, cross-adsorbedAntibody DyLight (R) 594 Conjugated used at a dilution of 1:100

Immunoprecipitation: ZCCHC8 Antibody [NB100-94995] - Detection of Human ZCCHC8 by Western Blot of Immunoprecipitates. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibody: Affinity purified rabbit anti-ZCCHC8 antibody NB100-94995 used for IP at 3 mcg/mg lysate. ZCCHC8 was also immunoprecipitated by rabbit anti-ZCCHC8 antibody NB100-94996, which recognizes a downstream epitope.

• Reactivity: Hu
• Applications: WB, ICC/IF, IHC, IP, Mycoplasma
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1.0 mg/ml

ZCCHC8 Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit ZCCHC8 Antibody - BSA Free (NB100-94995) is a polyclonal antibody validated for use in IHC, WB, ICC/IF and IP. Anti-ZCCHC8 Antibody: Cited in 5 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: The immunogen recognized by this antibody maps to a region between residue 1 and 50 of human zinc finger, CCHC domain containing 8 using the numbering given in entry NP_060082.2 (GeneID 55596).
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: ZCCHC8
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:1000 - 1:5000
  • Immunohistochemistry 1:2000 - 1:10000
  • Immunohistochemistry-Paraffin 1:2000 - 1:10000
  • Immunoprecipitation 2-5 ug/mg lysate
  • Knockdown Validated
  • Western Blot 1:100-1:2000
• Application Notes: Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections. ZCCHC8 antibody validated for WB from a verified customer review.
• Control:

  ZCCHC8 Overexpression Lysate

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: Tris-Citrate/Phosphate (pH 7.0 - 8.0)
• Preservative: 0.09% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for ZCCHC8 Antibody - BSA Free

• DKFZp434E2220
• zinc finger CCHC domain-containing protein 8
• zinc finger, CCHC domain containing 8

Background

ZCCHC8 is a zinc-finger protein that bears a zinc-binding motif composed of a CX2CX4HX4C sequence (where X can be any amino acid). ZCCHC8 also bears a PSP domain, a proline rich domain found in numerous splicesome associated proteins. ZCCHC8 has been identified as a component of the splicesome C complex and as a substrate of glycogen synthase kinase-3. It is suggested to play a role in the processing and metabolism of RNA.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for ZCCHC8 Antibody (NB100-94995)(6)

Publications using NB100-94995

• Kruse T, Garvanska DH, Varga JK et al. Substrate recognition principles for the PP2A-B55 protein phosphatase Science Advances 2024-10-04 [PMID: 39356758]
• Polák P, Garland W, Schmid M et al. Dual agonistic and antagonistic roles of ZC3H18 provides for co-activation of distinct nuclear RNA decay pathways bioRxiv 2023-05-10 [PMID: 37889751] (IP, Human)
• Ogami K, Richard P, Chen Y et al. An Mtr4/ZFC3H1 complex facilitates turnover of unstable nuclear RNAs to prevent their cytoplasmic transport and global translational repression. Gens & Dev. 2017-07-21 [PMID: 28733371]
• Chen Y Studies on RNA Regulation: From Enhancer RNAs to RBBP6 Isoform3 Thesis 2019-01-01
• Wu M, Karadoulama E, Lloret-Llinares M et al. The RNA exosome shapes the expression of key protein-coding genes Nucleic Acids Res. 2020-07-25 [PMID: 32710631] (WB, KD, Human)
• Wu G, Schmid M, Rib L et al. A Two-Layered Targeting Mechanism Underlies Nuclear RNA Sorting by the Human Exosome Cell Rep 2020-02-18 [PMID: 32075771] (WB, Human)

Reviews for ZCCHC8 Antibody (NB100-94995) (2)

Average Rating: 5

(Based on 2 reviews)

We have 2 reviews tested in 1 species: Human.

reviewed by: Verified Customer IP Human 02/11/2015

Summary

• Application: Immunoprecipitation
• Sample Tested: HeLa nuclear extract
• Species: Human
• Lot: A1

reviewed by: Verified Customer WB Human 02/11/2015

Summary

• Application: Western Blot
• Sample Tested: Hela whole cell lysate
• Species: Human
• Lot: A1

Bioinformatics

• Gene Symbol: ZCCHC8
• Entrez:
  • 55596(Human)
• Uniprot:
  • NP_060082.2(Human)