ZCCHC8 Antibody

Images

 
Western Blot: ZCCHC8 Antibody [NB100-94995] - Analysis of ZCCHC8 in HeLa whole cell lsyate using anti-ZCCHC8 antibody. Lane1: Control siRNA, Lane 2: HeLa cells treated with ZCCHC8 siRNA. Image from verified customer ...read more
Immunocytochemistry/ Immunofluorescence: ZCCHC8 Antibody [NB100-94995] - FFPE sections of human breast carcinoma. Antibody: Affinity purified rabbit anti-ZCCHC8 (A301-805A) used at a dilution of 1:5,000 (0.2ug/ml) and ...read more
Immunohistochemistry: ZCCHC8 Antibody [NB100-94995] - Sample: FFPE sections of human breast carcinoma. Antibody: Affinity purified rabbit anti- ZCCHC8 used at a dilution of 1:5,000 (0.2ug/ml) and 1:2,000 (0.5ug/ml). ...read more
Immunohistochemistry-Paraffin: ZCCHC8 Antibody [NB100-94995] - Section of human breast carcinoma. Antibody: Affinity purified rabbit anti-ZCCHC8 at a dilution of 1:250.
Immunoprecipitation: ZCCHC8 Antibody [NB100-94995] - Detection of Human ZCCHC8 by Western Blot of Immunoprecipitates. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibody: Affinity ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1.0 mg/ml
 

Independent Antibodies

   

Genetic Strategies

   

Order Details

ZCCHC8 Antibody Summary

Immunogen
The immunogen recognized by this antibody maps to a region between residue 1 and 50 of human zinc finger, CCHC domain containing 8 using the numbering given in entry NP_060082.2 (GeneID 55596).
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
ZCCHC8
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:100-1:2000
  • Immunocytochemistry/Immunofluorescence 1:1000 - 1:5000
  • Immunohistochemistry 1:2000 - 1:10000
  • Immunohistochemistry-Paraffin 1:10-1:500
  • Immunoprecipitation 2-5 mcg/mg lysate
Positive Control
ZCCHC8 Lysate (NBL1-17995)
Reviewed Applications
Read 2 Reviews rated 5
using
NB100-94995 in the following applications:

Publications
Read Publication using NB100-94995.

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
Tris-Citrate/Phosphate (pH 7.0 - 8.0)
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for ZCCHC8 Antibody

  • DKFZp434E2220
  • zinc finger CCHC domain-containing protein 8
  • zinc finger, CCHC domain containing 8

Background

ZCCHC8 is a zinc-finger protein that bears a zinc-binding motif composed of a CX2CX4HX4C sequence (where X can be any amino acid). ZCCHC8 also bears a PSP domain, a proline rich domain found in numerous splicesome associated proteins. ZCCHC8 has been identified as a component of the splicesome C complex and as a substrate of glycogen synthase kinase-3. It is suggested to play a role in the processing and metabolism of RNA.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for ZCCHC8 Antibody (NB100-94995)(1)

Reviews for ZCCHC8 Antibody (NB100-94995) (2) 52

Average Rating: 5
(Based on 2 reviews)
We have 2 reviews tested in 1 species: Human.
We have 2 reviews tested in 2 application: IP, WB.

Reviews using NB100-94995:
Filter by Applications
IP
(1)
WB
(1)
All Applications
Filter by Species
Human
(2)
All Species
Images Ratings Applications Species Date Details
Immunoprecipitation ZCCHC8 NB100-94995
Enlarge
reviewed by:
Anonymous
IP Human 02/11/2015
View

Summary

ApplicationImmunoprecipitation
Sample TestedHeLa nuclear extract
SpeciesHuman
LotA1

Blocking

Blocking Details5% skim milk/TBS, 1h at r.t.

Primary Anitbody

Dilution Ratio1:1000, over night at 4 degree celsius

Secondary Antibody

Secondary DescriptionRabbit IgG, HRP

Details

Detection NotesECL, 10sec-1min exposure
Immunoprecipitation Detailsprotein G agarose, incubate for 2h at 4C, wash 3 times with buffer containing 1% NP-40 and 150mM NaCl
Elution DescriptionSDS-PAGE sample buffer, boil for 5min
Western Blot ZCCHC8 NB100-94995
Enlarge
reviewed by:
Anonymous
WB Human 02/11/2015
View

Summary

ApplicationWestern Blot
Sample TestedHela whole cell lysate
SpeciesHuman
LotA1

Blocking

Blocking Details5% skim milk/TBS, 1h at r.t.

Primary Anitbody

Dilution Ratio1:1000, over night at 4 degree celsius

Secondary Antibody

Secondary DescriptionRabbit IgG, HRP
Secondary Concentration1:10000

Details

Detection NotesECL, 30sec-1min exposure

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for ZCCHC8 Antibody (NB100-94995) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Bioinformatics Tool for ZCCHC8 Antibody (NB100-94995)

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Blogs on ZCCHC8

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Calculators

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Recent Reviews

5
5
2
4
0
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2
0
1
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Anonymous
02/11/2015
Application: IP
Species: Human

Anonymous
02/11/2015
Application: WB
Species: Human

Bioinformatics

Gene Symbol ZCCHC8
Entrez
Uniprot