ZCCHC11/TUT4 Antibody

Western Blot: ZCCHC11/TUT4 Antibody [NBP1-42654] - Whole cell lysate from HeLa (5, 15 and 50 ug) and 293T (T; 50 ug) cells. Antibody used at 0.04 ug/ml.

• Reactivity: Hu
• Applications: WB, IP (-)
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 0.2 mg/ml

ZCCHC11/TUT4 Antibody Summary

• Description: Novus Biologicals Rabbit ZCCHC11/TUT4 Antibody (NBP1-42654) is a polyclonal antibody validated for use in WB. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: The immunogen for this product maps to a region between residue 1 and 50 of human zinc finger, CCHC domain containing 11 (NP_056084.1).
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: TUT4
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1:2000-1:10000
• Application Notes: Immunoprecipitation Not recommended

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: TBS and 0.1% BSA
• Preservative: 0.09% Sodium Azide
• Concentration: 0.2 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for ZCCHC11/TUT4 Antibody

• DKFZp779C1943
• EC 2.7.7.52
• KIAA0191FLJ42878
• PAP associated domain containing 3
• PAPD3
• terminal uridylyltransferase 4
• TUT4
• TUTase 4
• Zinc finger CCHC domain-containing protein 11
• zinc finger, CCHC domain containing 11

Background

Uridylyltransferase that acts as a suppressor of microRNA (miRNA) biogenesis by specifically mediating the terminal uridylation of some miRNAs. Catalyzes the 3' uridylation of precursor let-7 (pre-let-7), a miRNA precursor. Uridylated pre-let-7 miRNAs fail to be processed by Dicer and undergo degradation. Degradation of pre-let-7 contributes to the maintenance of embryonic stem (ES) cells and is required for ES cells to maintain pluripotency. Does not bind RNA by itself, recruited to pre-let-7 miRNAs via its interaction with LIN28 and LIN28B. Also catalyzes the 3' uridylation of miR-26A, a miRNA that represses IL6 transcript, leading to abrogate IL6 transcript repression and promote cytokine expression. May also suppress Toll-like receptor-induced NF-kappa-B activity via binding to T2BP. Does not play a role in replication-dependent histone mRNA degradation

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: TUT4
• Entrez:
  • 23318(Human)
• Uniprot:
  • Q5TAX3(Human)