ZBTB7A/Pokemon Antibody (466407)

Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, MCF-7 human breast cancer cell line, HepG2 human hepatocellular carcinoma cell line, and NIH-3T3 mouse embryonic fibroblast cell line. PVDF Membrane was probed with 1 µg/mL of Mouse Anti-Human/Mouse ZBTB7A/Pokemon Monoclonal Antibody (Catalog # MAB3496) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for ZBTB7A/Pokemon at approximately 75 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 10.

• Reactivity: Hu, Mu
• Applications: WB
• Clone: 466407
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: LYOPH

ZBTB7A/Pokemon Antibody (466407) Summary

• Immunogen: E. coli-derived recombinant human ZBTB7A/Pokemon
  Met349-Val476
  Accession # O95365
• Specificity: Detects human and mouse ZBTB7A/Pokemon in Western blots.
• Source: N/A
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: ZBTB7A
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for ZBTB7A/Pokemon Antibody (466407)

• DKFZp547O146
• Factor binding IST protein 1
• Factor that binds to inducer of short transcripts protein 1
• FBI-1
• FBI-1POK erythroid myeloid ontogenic factor
• FBI1TTF-I-interacting peptide 21
• HIV-1 inducer of short transcripts binding protein
• Leukemia/lymphoma-related factor
• LRF
• LRFHIV-1 1st-binding protein 1
• lymphoma related factor
• MGC99631
• Pokemon
• TIP21
• ZBTB7A
• ZBTB7zinc finger and BTB domain containing 7
• zinc finger and BTB domain containing 7A
• zinc finger and BTB domain containing 7A, HIV-1 inducer of short transcriptsbinding protein
• zinc finger and BTB domain-containing protein 7A
• Zinc finger protein 857A
• ZNF857APOZ and Krueppel erythroid myeloid ontogenic factor

Background

ZBTB7A (zinc finger and BTB domain-containing 7A), also known as Pokemon, FBI-1 in human, LRF in mouse, and OCZF in rat, is a widely expressed nuclear protein that is overexpressed in many human cancers. It is a transcriptional repressor of ARF, c-fos, c-myc, Rb and others, including many extracellular matrix genes. These targets play important roles, both in normal differentiation and oncogenesis. Human ZBTB7A/Pokemon is a 584 amino acid (aa) protein with a BTB-POZ domain (aa 24‑131) and four zinc finger repeats (aa 382‑484). Human ZBTB7A/Pokemon shares 98% aa identity with mouse and rat within aa 349‑476.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for ZBTB7A/Pokemon Antibody (MAB3496)(1)

Publication using MAB3496

• Pamela Himadewi, Xue Qing David Wang, Fan Feng, Haley Gore, Yushuai Liu, Lei Yu et al. 3′HS1 CTCF binding site in human beta -globin locus regulates fetal hemoglobin expression eLife 2021-09-29 [PMID: 34585664]

Bioinformatics

• Gene Symbol: ZBTB7A
• Uniprot:
  • O95365()