ZAR1 Overexpression Lysate

Images

 
Western Blot: ZAR1 Overexpression Lysate (Adult Normal) [NBL1-17958] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for ZAR1.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB

Order Details

ZAR1 Overexpression Lysate Summary

Description

ZAR1 Transient Overexpression Lysate


Expression Host: HEK293T

Plasmid: RC221731

Accession#: NM_175619

Protein Tag: C-MYC/DDK
Gene
ZAR1

Applications/Dilutions

Dilutions
  • Western Blot
Application Notes
This product is intended for use as a positive control in Western Blot.

You will receive 1 vial of lysate (100ug), 1 vial of empty vector negative control (100ug), and 1 vial of 2xSDS sample buffer (250ul).
Theoretical MW
45.7 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
RIPA buffer

Lysate Details for Array

Type
Overexpression

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for ZAR1 Overexpression Lysate

  • DKFZp686J1831
  • Zinc finger protein 265FLJ41119
  • zinc finger, RAN-binding domain containing 2
  • Zinc finger, splicing
  • zinc-finger protein 265
  • zinc-finger, splicing
  • ZIS1
  • ZIS2
  • ZISDKFZp686N09117
  • ZNF265zinc finger Ran-binding domain-containing protein 2

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

Customers Who Viewed This Item Also Viewed...

739-G9/CF
Species: Mu
Applications: BA
NBP1-76289
Species: Hu, Mu, Rt
Applications: ELISA, ICC/IF, IHC, IHC-P, WB
NB500-181
Species: Dr, Hu, Ma, Mu, Pl, Po, Rt
Applications: IB, ICC/IF, IP, WB
PP-A8620A-00
Species: Hu, Mu
Applications: DirELISA, IHC, IP, WB
5096-BM
Species: Hu
Applications: BA
NBP1-82558
Species: Hu
Applications: ICC/IF, IHC, IHC-P, WB
NBP1-87835
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, IHC-P, KD, WB
NBP1-21367
Species: Hu, Mu
Applications: ICC/IF, IHC, IHC-P, IP, WB
NB500-174
Species: Hu, Ma, Mar, Mu, Pm, Rt
Applications: ICC/IF, IHC, IHC-Fr, IHC-P, IP, Simple Western, WB
AF4820
Species: Hu
Applications: IHC, WB
H00057093-M01
Species: Hu
Applications: ELISA, IHC, IHC-P, S-ELISA, WB
AF6000
Species: Hu, Mu
Applications: IHC, Simple Western, WB
NBP2-67451
Species: Hu, Mu, Rt
Applications: Flow, ICC/IF, IHC, IHC-P, IP, KO, WB
NB100-56519
Species: Bv, Hu, Mu, Po, Rt, Sh
Applications: ChIP, CyTOF-ready, Flow-IC, Flow, ICC/IF, IHC-FrFl, IHC, IHC-Fr, IHC-P, IP, KD, Simple Western, WB
NBP2-13673
Species: Hu
Applications: ICC/IF, IHC, IHC-P
NBP1-86265
Species: Hu
Applications: IHC, IHC-P, WB
NBP1-51843
Species: Hu, Mu
Applications: ICC/IF, IHC, IHC-P, Simple Western, WB
NBP1-62315
Species: Hu
Applications: WB

Publications for ZAR1 Lysate (NBL1-17958) (0)

There are no publications for ZAR1 Lysate (NBL1-17958).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for ZAR1 Lysate (NBL1-17958) (0)

There are no reviews for ZAR1 Lysate (NBL1-17958). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for ZAR1 Lysate (NBL1-17958) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Additional ZAR1 Products

Array NBL1-17958

Bioinformatics Tool for ZAR1 Lysate (NBL1-17958)

Discover related pathways, diseases and genes to ZAR1 Lysate (NBL1-17958). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for ZAR1 Lysate (NBL1-17958)

Discover more about diseases related to ZAR1 Lysate (NBL1-17958).
 

Pathways for ZAR1 Lysate (NBL1-17958)

View related products by pathway.

PTMs for ZAR1 Lysate (NBL1-17958)

Learn more about PTMs related to ZAR1 Lysate (NBL1-17958).

Blogs on ZAR1

There are no specific blogs for ZAR1, but you can read our latest blog posts.

Customers Who Bought This Also Bought

Eg5 Antibody
NB500-181

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our ZAR1 Overexpression Lysate and receive a gift card or discount.

Bioinformatics

Gene Symbol ZAR1