Yes Antibody - BSA Free

Images

 
Western Blot: Yes Antibody [NBP1-31297] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with c-Yes antibody diluted at 1:500.
Immunocytochemistry/ Immunofluorescence: Yes Antibody [NBP1-31297] - Analysis of methanol-fixed HeLa, using antibody at 1:200 dilution.
Western Blot: Yes Antibody [NBP1-31297] - Sample (30 ug of whole cell lysate) A: A431 7.5% SDS PAGE; antibody diluted at 1:1000.
Western Blot: Yes Antibody [NBP1-31297] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with c-Yes antibody diluted at 1:500.

Product Details

Summary
Reactivity Hu, Mu, Rt, Bv, RMSpecies Glossary
Applications WB, ICC/IF, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Format
BSA Free

Order Details

Yes Antibody - BSA Free Summary

Description
Novus Biologicals Rabbit Yes Antibody - BSA Free (NBP1-31297) is a polyclonal antibody validated for use in IHC, WB and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
Immunogen
Recombinant protein encompassing a sequence within the center region of human Yes. The exact sequence is proprietary.
Localization
Cytoplasm, cytosol
Predicted Species
Bovine (100%), Rhesus Macaque (100%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
YES1
Purity
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 1:100-1:1000
  • Immunohistochemistry
  • Immunohistochemistry-Frozen Assay dependent
  • Western Blot 1:500-1:3000
Theoretical MW
61 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
PBS, 20% Glycerol
Preservative
0.01% Thimerosal
Purity
Antigen Affinity-purified

Alternate Names for Yes Antibody - BSA Free

  • cellular yes-1 protein
  • c-yes
  • EC 2.7.10
  • EC 2.7.10.2
  • HsT441
  • P61-YES
  • Proto-oncogene c-Yes
  • proto-oncogene tyrosine-protein kinase Yes
  • v-yes-1 Yamaguchi sarcoma viral oncogene homolog 1
  • Yamaguchi sarcoma oncogene
  • Yes
  • YES1

Background

This gene is the cellular homolog of the Yamaguchi sarcoma virus oncogene. The encoded protein has tyrosine kinase activity and belongs to the src family of proteins. This gene lies in close proximity to thymidylate synthase gene on chromosome 18, and a corresponding pseudogene has been found on chromosome 22.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

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Product General Protocols

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Video Protocols

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ICC/IF Video Protocol

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Bioinformatics

Gene Symbol YES1