Genetic Strategies: Knockdown Validated: YB1 Antibody [NBP1-97572] - Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and YB-1 knockout (KO) HeLa cell line. PVDF membrane ...read more
Western Blot: YB1 Antibody [NBP1-97572] - Western blot analysis of YB1 expression in 1) HeLa and 2) NTera2 whole cell lysates using NBP1-97572.
Immunocytochemistry/ Immunofluorescence: YB1 Antibody [NBP1-97572] - The YB1 antibody was tested in HeLa cells with Dylight 488 (green). Nuclei and alpha-tubulin (NB100-690) were counterstained with DAPI (blue) and ...read more
Immunohistochemistry: YB1 Antibody [NBP1-97572] - IHC staining of YB1 in mouse testis using DAB with hematoxylin counterstain.
Simple Western: YB1 Antibody [NBP1-97572] - Simple Western lane view shows a specific band for YB1 in 0.5 mg/ml of HeLa lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation ...read more
Hu, Mu, Rt, Rb, Bv, Ch, RbSpecies Glossary
WB, Simple Western, ICC/IF, IHC, IHC-P, In vivo, IP, KD
This YB1 polyclonal antibody is useful for Immunocytochemistry/Immunofluorescence, Western blot, Immunohistochemistry on paraffin embedded sections and Immunoprecipitation. In ICC/IF, cytoplasmic staining was observed in HeLa cells. In WB, a band is seen at approx. 50 kDa representing YB1. In IHC-P, staining was observed in the cytoplasm, nucleus, and nucleoli of mouse testis, brain and kidney tissues. Prior to immunostaining paraffin tissues, antigen retrieval with sodium citrate buffer (pH 6.0) is recommended.
In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. Use in vivo reported in scientific literature (PMID: 11447123).
50 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Rabbit reactivity reported in scientific literature (Bonenfant et al).
Packaging, Storage & Formulations
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
0.05% Sodium Azide
Alternate Names for YB1 Antibody
class II, Y box-binding protein I
DBPB CCAAT-binding transcription factor I subunit A
Enhancer factor I subunit A
NSEP1 Y-box-binding protein 1
nuclease-sensitive element-binding protein 1
Y box binding protein 1
YB1 DNA-binding protein B
YB-1 Y-box transcription factor
YB1, also known as YBX1 (Y box binding protein 1) or DbpB (DNA binding protein B), is a transcription factor that binds single-stranded DNA within the reverse CCAAT promoter element. YB1 is found abundantly in the cytoplasm and nucleus of many cell types. It binds to cytoplasmic mRNA and participates in translation regulation. Additionally, it may play a role in DNA repair. Current research has shown that YB1 is overexpressed in many different types of cancerous tissues including non-small cell lung carcinoma, breast cancer, colorectal cancer, and ovarian adenocarcinomas.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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