YB1 Antibody

Images

 
Western Blot: YB1 Antibody [NBP1-97572] - Western blot analysis of YB1 expression in 1) HeLa and 2) NTera2 whole cell lysates using NBP1-97572.
Immunocytochemistry/ Immunofluorescence: YB1 Antibody [NBP1-97572] - The YB1 antibody was tested in HeLa cells with Dylight 488 (green). Nuclei and alpha-tubulin (NB100-690) were counterstained with DAPI (blue) and ...read more
Immunohistochemistry: YB1 Antibody [NBP1-97572] - IHC staining of YB1 in mouse testis using DAB with hematoxylin counterstain.
Simple Western: YB1 Antibody [NBP1-97572] - Simple Western lane view shows a specific band for YB1 in 0.5 mg/ml of HeLa lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, Bv, Ch, RbSpecies Glossary
Applications WB, Simple Western, ICC/IF, IHC, IHC-P, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

YB1 Antibody Summary

Immunogen
A C-terminal peptide of human YB1. [Swiss-Prot# P67809]
Localization
Cytoplasm. Nucleus. Cytoplasmic granule. Secreted.
Predicted Species
Bovine (100%), Rabbit (100%), Chicken (93%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
YBX1
Purity
Unpurified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:5000-1:10000
  • Simple Western 1:500
  • Immunocytochemistry/Immunofluorescence 1:100-1:200
  • Immunohistochemistry 1:50-1:200
  • Immunohistochemistry-Paraffin 1:50-1:200
  • Immunoprecipitation 1:10-1:100
Application Notes
This YB1 polyclonal antibody is useful for Immunocytochemistry/Immunofluorescence, Western blot, Immunohistochemistry on paraffin embedded sections and Immunoprecipitation. In ICC/IF, cytoplasmic staining was observed in HeLa cells. In WB, a band is seen at approx. 50 kDa representing YB1. In IHC-P, staining was observed in the cytoplasm, nucleus, and nucleoli of mouse testis, brain and kidney tissues. Prior to immunostaining paraffin tissues, antigen retrieval with sodium citrate buffer (pH 6.0) is recommended.

In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue.
The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Theoretical MW
50 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Control
HeLa Lysate (NB800-PC1)
NTERA-2 Lysate (NBP1-44968)
YB1 Lysate (NBP2-64988)
Publications
Read Publication using
NBP1-97572 in the following applications:

Reactivity Notes

Human, Mouse and Rat. Based on immunogen's sequence homology, this antibody is predicted to react with Bovine (100%), Rabbit (100%), Chicken (93%) and Xenopus (87%).

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
Whole antisera
Preservative
0.05% Sodium Azide
Purity
Unpurified

Alternate Names for YB1 Antibody

  • BP-8
  • CBF-A
  • class II, Y box-binding protein I
  • CSDA2
  • CSDB
  • DBPB CCAAT-binding transcription factor I subunit A
  • EFI-A
  • Enhancer factor I subunit A
  • MDR-NF1
  • MGC104858
  • MGC110976
  • MGC117250
  • NSEP1 Y-box-binding protein 1
  • nuclease-sensitive element-binding protein 1
  • Y box binding protein 1
  • YB1 DNA-binding protein B
  • YB-1 Y-box transcription factor
  • YBX1

Background

YB1, also known as YBX1 (Y box binding protein 1) or DbpB (DNA binding protein B), is a transcription factor that binds single-stranded DNA within the reverse CCAAT promoter element. YB1 is found abundantly in the cytoplasm and nucleus of many cell types. It binds to cytoplasmic mRNA and participates in translation regulation. Additionally, it may play a role in DNA repair. Current research has shown that YB1 is overexpressed in many different types of cancerous tissues including non-small cell lung carcinoma, breast cancer, colorectal cancer, and ovarian adenocarcinomas.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for YB1 Antibody (NBP1-97572)(1)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 2 applications: IP, In Vivo.


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In Vivo
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Product General Protocols

View specific protocols for YB1 Antibody (NBP1-97572): Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Blogs on YB1.

New Techniques Using Phosphoserine Antibodies
Phosphoserine, the phosphorylated modification of the amino acid serine, is a central post-translational modification within a cell for many biological and biomedical processes. The phosphorylation of specifically four residue types - histidine, serin...  Read full blog post.

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Bioinformatics

Gene Symbol YBX1
Entrez
Uniprot