YB1 Antibody


Western Blot: YB1 Antibody [NBP1-97572] - Western blot analysis of YB1 expression in 1) HeLa and 2) NTera2 whole cell lysates using NBP1-97572.
Immunocytochemistry/ Immunofluorescence: YB1 Antibody [NBP1-97572] - The YB1 antibody was tested in HeLa cells with Dylight 488 (green). Nuclei and alpha-tubulin (NB100-690) were counterstained with DAPI (blue) and ...read more
Immunohistochemistry: YB1 Antibody [NBP1-97572] - IHC staining of YB1 in mouse testis using DAB with hematoxylin counterstain.
Simple Western: YB1 Antibody [NBP1-97572] - Simple Western lane view shows a specific band for YB1 in 0.5 mg/ml of HeLa lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation ...read more

Product Details

Reactivity Hu, Mu, Rt, Bv, Ch, RbSpecies Glossary
Applications WB, Simple Western, ICC/IF, IHC, IHC-P, IP

Order Details

YB1 Antibody Summary

A C-terminal peptide of human YB1. [Swiss-Prot# P67809]
Cytoplasm. Nucleus. Cytoplasmic granule. Secreted.
Predicted Species
Bovine (100%), Rabbit (100%), Chicken (93%). Backed by our 100% Guarantee.
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.


  • Western Blot 1:5000-1:10000
  • Simple Western 1:500
  • Immunocytochemistry/Immunofluorescence 1:100-1:200
  • Immunohistochemistry 1:50-1:200
  • Immunohistochemistry-Paraffin 1:50-1:200
  • Immunoprecipitation 1:10-1:100
Application Notes
This YB1 polyclonal antibody is useful for Immunocytochemistry/Immunofluorescence, Western blot, Immunohistochemistry on paraffin embedded sections and Immunoprecipitation. In ICC/IF, cytoplasmic staining was observed in HeLa cells. In WB, a band is seen at approx. 50 kDa representing YB1. In IHC-P, staining was observed in the cytoplasm, nucleus, and nucleoli of mouse testis, brain and kidney tissues. Prior to immunostaining paraffin tissues, antigen retrieval with sodium citrate buffer (pH 6.0) is recommended.

In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue.
The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Theoretical MW
50 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
HeLa Lysate (NB800-PC1)
NTERA-2 Lysate (NBP1-44968)
YB1 Lysate (NBP2-64988)
Read Publication using
NBP1-97572 in the following applications:

Reactivity Notes

Human, Mouse and Rat. Based on immunogen's sequence homology, this antibody is predicted to react with Bovine (100%), Rabbit (100%), Chicken (93%) and Xenopus (87%).

Packaging, Storage & Formulations

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Whole antisera
0.05% Sodium Azide

Alternate Names for YB1 Antibody

  • BP-8
  • CBF-A
  • class II, Y box-binding protein I
  • CSDA2
  • CSDB
  • DBPB CCAAT-binding transcription factor I subunit A
  • EFI-A
  • Enhancer factor I subunit A
  • MDR-NF1
  • MGC104858
  • MGC110976
  • MGC117250
  • NSEP1 Y-box-binding protein 1
  • nuclease-sensitive element-binding protein 1
  • Y box binding protein 1
  • YB1 DNA-binding protein B
  • YB-1 Y-box transcription factor
  • YBX1


YB1, also known as YBX1 (Y box binding protein 1) or DbpB (DNA binding protein B), is a transcription factor that binds single-stranded DNA within the reverse CCAAT promoter element. YB1 is found abundantly in the cytoplasm and nucleus of many cell types. It binds to cytoplasmic mRNA and participates in translation regulation. Additionally, it may play a role in DNA repair. Current research has shown that YB1 is overexpressed in many different types of cancerous tissues including non-small cell lung carcinoma, breast cancer, colorectal cancer, and ovarian adenocarcinomas.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

Species: Hu
Applications: WB, ELISA, ICC/IF, IHC-P, RNAi
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P
Species: Hu, Mu, Rt, Ca, Mk
Applications: WB, ELISA, IHC, IHC-P
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-Fr, IHC-P
Species: Hu, Mu, Rt
Applications: WB, ChIP, ICC/IF, IHC, IHC-P
Species: Hu
Applications: ICC/IF, IHC, IHC-P
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P
Species: Hu, Mu, Rt, Ye, Xp(-)
Applications: WB, ELISA, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, CyTOF-ready, Flow-IC
Species: Hu, Mu, Rt
Applications: WB, ChIP, ICC/IF, IHC, IHC-P, IP, ChIP
Species: Hu
Applications: WB, Flow, IHC, IHC-P
Species: Hu
Applications: WB, IHC, IHC-P
Species: Hu
Applications: WB, Flow, IHC, IP, CyTOF-ready, ELISA(Cap), ELISA(Det), ICC, ELISA(Sta)
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P

Publications for YB1 Antibody (NBP1-97572)(1)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 2 applications: IP, In Vivo.

Filter By Application
In Vivo
All Applications
Filter By Species
All Species

Reviews for YB1 Antibody (NBP1-97572) (0)

There are no reviews for YB1 Antibody (NBP1-97572). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen
  • 1st to review with an image -- $50/€35/£30/$50 CAD/¥300 Yuan/¥5000 Yen

Product General Protocols

View specific protocols for YB1 Antibody (NBP1-97572): Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for YB1 Antibody (NBP1-97572) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Control Lysate(s)

Secondary Antibodies


Isotype Controls

Additional Array Products

Bioinformatics Tool for YB1 Antibody (NBP1-97572)

Discover related pathways, diseases and genes to YB1 Antibody (NBP1-97572). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for YB1 Antibody (NBP1-97572)

Discover more about diseases related to YB1 Antibody (NBP1-97572).

Pathways for YB1 Antibody (NBP1-97572)

View related products by pathway.

PTMs for YB1 Antibody (NBP1-97572)

Learn more about PTMs related to YB1 Antibody (NBP1-97572).

Research Areas for YB1 Antibody (NBP1-97572)

Find related products by research area.

Blogs on YB1.

New Techniques Using Phosphoserine Antibodies
Phosphoserine, the phosphorylated modification of the amino acid serine, is a central post-translational modification within a cell for many biological and biomedical processes. The phosphorylation of specifically four residue types - histidine, serin...  Read full blog post.

Customers Who Bought This Also Bought

Contact Information

Product PDFs


Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.


Review this Product

Be the first to review our YB1 Antibody and receive a gift card or discount.


Gene Symbol YBX1