| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-74905 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-98908-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-08774 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-58086-25ul | |||
![Western Blot: YB1 Antibody [NBP1-89945] - Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and YB-1 knockout (KO) HeLa cell line. PVDF membrane was probed with 0.4 ug/ml of Rabbit Anti-Human YB-1 Polyclonal Antibody (Catalog # NBP1-89945) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog #HAF008). Specific band was detected for YB-1 at approximately 49 kDa (as indicated) in the parental HeLa cell line, but is not detectable in the knockout HeLa cell line. This experiment was conducted under reducing conditions.](http://images.novusbio.com/fullsize/YB1-Antibody-Knockdown-Validated-NBP1-89945-img0019.jpg "<b>Genetic Strategies Validation. </b>Western Blot: YB1 Antibody [NBP1-89945] - Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and YB-1 knockout (KO) HeLa cell line. PVDF membrane was probed with 0.4 ug/ml of Rabbit Anti-Human YB-1 Polyclonal Antibody (Catalog # NBP1-89945) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog #HAF008). Specific band was detected for YB-1 at approximately 49 kDa (as indicated) in the parental HeLa cell line, but is not detectable in the knockout HeLa cell line. This experiment was conducted under reducing conditions.")
![<b>Genetic Strategies Validation. </b>Western Blot: YB1 Antibody [NBP1-89945] - Analysis in HEK293 cells transfected with control siRNA, target specific siRNA probe #1 and #2, using Anti-YBX1 antibody. Remaining relative intensity is presented. Loading control: Anti-PPIB.](http://images.novusbio.com/fullsize/YB1-Antibody-Western-Blot-NBP1-89945-img0013.jpg)
![Immunocytochemistry/Immunofluorescence: YB1 Antibody [NBP1-89945] - Staining of human cell line U-251 MG shows localization to cytosol. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/YB1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-89945-img0011.jpg)
![Immunohistochemistry-Paraffin: YB1 Antibody [NBP1-89945] - Staining of human gastrointestinal shows strong cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/YB1-Antibody-Immunohistochemistry-Paraffin-NBP1-89945-img0018.jpg)
![Western Blot: YB1 Antibody [NBP1-89945] - Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.](http://images.novusbio.com/fullsize/YB1-Antibody-Western-Blot-NBP1-89945-img0008.jpg)
![Immunohistochemistry-Paraffin: YB1 Antibody [NBP1-89945] - Staining of human liver shows very weak cytoplasmic positivity in hepatocytes.](http://images.novusbio.com/fullsize/YB1-Antibody-Immunohistochemistry-Paraffin-NBP1-89945-img0015.jpg)
![Immunohistochemistry-Paraffin: YB1 Antibody [NBP1-89945] - Staining of human lymph node shows moderate cytoplasmic positivity in germinal center cells.](http://images.novusbio.com/fullsize/YB1-Antibody-Immunohistochemistry-Paraffin-NBP1-89945-img0016.jpg)
![Immunohistochemistry-Paraffin: YB1 Antibody [NBP1-89945] - Staining of human testis shows strong cytoplasmic positivity in cells in seminiferous ducts.](http://images.novusbio.com/fullsize/YB1-Antibody-Immunohistochemistry-Paraffin-NBP1-89945-img0017.jpg)
![Western Blot: YB1 Antibody (9J7M4) [NBP3-16213] - Analysis of extracts of various cell lines, using YB1 Rabbit mAb (NBP3-16213) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.](http://images.novusbio.com/fullsize/YB1-Antibody-9J7M4-Western-Blot-NBP3-16213-img0008.jpg "Western Blot: YB1 Antibody (9J7M4) [NBP3-16213] - Analysis of extracts of various cell lines, using YB1 Rabbit mAb (NBP3-16213) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.")
![Western Blot: YB1 Antibody (9J7M4) [NBP3-16213] - Analysis of extracts of HeLa cells, using YB1 Rabbit mAb (NBP3-16213) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.](http://images.novusbio.com/fullsize/YB1-Antibody-9J7M4-Western-Blot-NBP3-16213-img0007.jpg)
![Immunoprecipitation: YB1 Antibody (9J7M4) [NBP3-16213] - Immunoprecipitation of YB1 from 200 ug extracts of HeLa cells was performed using 0.5 ug of YB1 Rabbit mAb . Rabbit IgG isotype control was used to precipitate the Control IgG sample. IP samples were eluted with 1X Laemmli Buffer. The Input lane represents 10% of the total input. Western blot analysis of immunoprecipitates was conducted using YB1 Rabbit mAb at a dilution of 1:1000.](http://images.novusbio.com/fullsize/nbp3-16213_rabbit-yb1-mab-9j7m4-5220251255573.jpg)
![Immunocytochemistry/ Immunofluorescence: YB1 Antibody (9J7M4) [NBP3-16213] - Confocal imaging of NIH/3T3 cells using YB1 Rabbit mAb followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L).The cells were counterstained with alpha-Tubulin Mouse mAb followed by incubation with ABflo(R) 488-conjugated Goat Anti-Mouse IgG (H+L) Ab (Green).DAPI was used for nuclear staining (Blue). Objective: 100x.](http://images.novusbio.com/fullsize/nbp3-16213_rabbit-yb1-mab-9j7m4-52202511361248.jpg)
![Immunohistochemistry: YB1 Antibody (9J7M4) [NBP3-16213] - Immunohistochemistry analysis of paraffin-embedded Human thyroid cancer using YB1 Rabbit mAb at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-16213_rabbit-yb1-mab-9j7m4-5220251215454.jpg)
![Immunohistochemistry: YB1 Antibody (9J7M4) [NBP3-16213] - Immunohistochemistry analysis of paraffin-embedded Human colon using YB1 Rabbit mAb at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-16213_rabbit-yb1-mab-9j7m4-52202511374130.jpg)
![Immunohistochemistry: YB1 Antibody (9J7M4) [NBP3-16213] - Immunohistochemistry analysis of paraffin-embedded Rat kidney using YB1 Rabbit mAb at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-16213_rabbit-yb1-mab-9j7m4-52202511411489.jpg)
![Immunohistochemistry: YB1 Antibody (9J7M4) [NBP3-16213] - Immunohistochemistry analysis of paraffin-embedded Rat colon using YB1 Rabbit mAb at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-16213_rabbit-yb1-mab-9j7m4-5220251140277.jpg)
![Immunohistochemistry: YB1 Antibody (9J7M4) [NBP3-16213] - Immunohistochemistry analysis of paraffin-embedded Mouse kidney using YB1 Rabbit mAb at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-16213_rabbit-yb1-mab-9j7m4-52202511392221.jpg)
![Immunocytochemistry/ Immunofluorescence: YB1 Antibody (9J7M4) [NBP3-16213] - Confocal imaging of U-2 OS cells using YB1 Rabbit mAb followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L).The cells were counterstained with alpha-Tubulin Mouse mAb followed by incubation with ABflo(R) 488-conjugated Goat Anti-Mouse IgG (H+L) Ab (Green).DAPI was used for nuclear staining (Blue). Objective: 100x.](http://images.novusbio.com/fullsize/nbp3-16213_rabbit-yb1-mab-9j7m4-52202511374198.jpg)
![Immunocytochemistry/ Immunofluorescence: YB1 Antibody (9J7M4) [NBP3-16213] - Confocal imaging of C6 cells using YB1 Rabbit mAb followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L).The cells were counterstained with alpha-Tubulin Mouse mAb followed by incubation with ABflo(R) 488-conjugated Goat Anti-Mouse IgG (H+L) Ab (Green).DAPI was used for nuclear staining (Blue). Objective: 100x.](http://images.novusbio.com/fullsize/nbp3-16213_rabbit-yb1-mab-9j7m4-52202511402797.jpg)
![Western Blot: YB1 Antibody (7B4) [NBP2-46381] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY YB1.](http://images.novusbio.com/fullsize/YB1-Antibody-7B4-Western-Blot-NBP2-46381-img0005.jpg "Western Blot: YB1 Antibody (7B4) [NBP2-46381] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY YB1.")
![Immunohistochemistry: YB1 Antibody (7B4) [NBP2-46381] - Analysis of Human lymphoma tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/YB1-Antibody-7B4-Immunohistochemistry-NBP2-46381-img0004.jpg)
![Immunohistochemistry: YB1 Antibody (7B4) [NBP2-46381] - Analysis of Adenocarcinoma of Human colon tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/YB1-Antibody-7B4-Immunohistochemistry-NBP2-46381-img0001.jpg)
![Immunohistochemistry: YB1 Antibody (7B4) [NBP2-46381] - Analysis of Carcinoma of Human kidney tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/YB1-Antibody-7B4-Immunohistochemistry-NBP2-46381-img0002.jpg)
![Immunohistochemistry: YB1 Antibody (7B4) [NBP2-46381] - Analysis of Adenocarcinoma of Human ovary tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/YB1-Antibody-7B4-Immunohistochemistry-NBP2-46381-img0003.jpg)
![SDS-Page: Recombinant Human YB1 Protein [NBP2-30101] - 15% SDS-Page(3ug)](http://images.novusbio.com/fullsize/Recombinant-Human-YB1-Protein-SDS-Page-NBP2-30101-img0002.jpg "SDS-Page: Recombinant Human YB1 Protein [NBP2-30101] - 15% SDS-Page(3ug)")
![Immunohistochemistry;YB1 Antibody[NBP3-29773]Detection of human YB1 by immunohistochemistry. Samples: FFPE sections of human ovarian carcinoma. Antibody: Affinity purified rabbit anti-YB1 used at a dilution of 1:5,000 . Detection: DAB](http://images.novusbio.com/fullsize/nbp3-29785_rabbit-yb1-pab-78202410153552.jpg "Immunohistochemistry;YB1 Antibody[NBP3-29773]Detection of human YB1 by immunohistochemistry. Samples: FFPE sections of human ovarian carcinoma. Antibody: Affinity purified rabbit anti-YB1 used at a dilution of 1:5,000 . Detection: DAB")
![Immunohistochemistry;YB1 Antibody[NBP3-29785]Detection of mouse YB1 by immunohistochemistry. Sample: FFPE section of mouse renal cell carcinoma. Antibody: Affinity purified rabbit anti-YB1 used at a dilution of 1:5,000 . Detection: DAB](http://images.novusbio.com/fullsize/nbp3-29785_rabbit-yb1-pab-78202410372636.jpg)
![Western Blot: YB1 Antibody (S03-6G4) [NBP3-19806] - Western blot detection of YB1 in Jurkat, Hela cell lysates using NBP3-19806 (1:1000 diluted). Predicted band size: 36kDa. Observed band size: 49kDa.](http://images.novusbio.com/fullsize/YB1-Antibody-S03-6G4-Western-Blot-NBP3-19806-img0002.jpg "Western Blot: YB1 Antibody (S03-6G4) [NBP3-19806] - Western blot detection of YB1 in Jurkat, Hela cell lysates using NBP3-19806 (1:1000 diluted). Predicted band size: 36kDa. Observed band size: 49kDa.")
![Immunohistochemistry-Paraffin: YB1 Antibody (S03-6G4) [NBP3-19806] - Immunohistochemistry of YB1 in paraffin-embedded Human lung cancer tissue using NBP3-19806 at dilution 1/50](http://images.novusbio.com/fullsize/YB1-Antibody-S03-6G4-Immunohistochemistry-Paraffin-NBP3-19806-img0001.jpg)
![Immunohistochemistry:[NBP3-30203]-Detection of mouse YB1 by immunohistochemistry. Sample: FFPE section of mouse teratoma. Antibody: Affinity purified rabbit anti-YB1 used at a dilution of 1: 1,000. Detection: DAB](http://images.novusbio.com/fullsize/nbp3-30203_rabbit-yb1-pab-2312025128882.jpg "Immunohistochemistry:[NBP3-30203]-Detection of mouse YB1 by immunohistochemistry. Sample: FFPE section of mouse teratoma. Antibody: Affinity purified rabbit anti-YB1 used at a dilution of 1: 1,000. Detection: DAB")
![Western Blot: YB1 Antibody [NBP2-98908] - Anti-YB1 rabbit polyclonal antibody at 1:500 dilution. Lane A: HeLa Whole Cell Lysate Lane B: SW480 Whole Cell Lysate Lane C: A549 Whole Cell Lysate Lane D: MCF7 Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 36 kDa. Observed band size: 36 kDa](http://images.novusbio.com/fullsize/YB1-Antibody-Western-Blot-NBP2-98908-img0003.jpg "Western Blot: YB1 Antibody [NBP2-98908] - Anti-YB1 rabbit polyclonal antibody at 1:500 dilution. Lane A: HeLa Whole Cell Lysate Lane B: SW480 Whole Cell Lysate Lane C: A549 Whole Cell Lysate Lane D: MCF7 Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 36 kDa. Observed band size: 36 kDa")
![Immunocytochemistry/Immunofluorescence: YB1 Antibody [NBP2-98908] - Immunofluorescence staining of YB1 in HeLa cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS, blocked with 10% serum, and incubated with rabbit anti-Human YB1 polyclonal antibody (dilution ratio 1:200) at 4C overnight. Then cells were stained with the Alexa Fluor(R)488-conjugated Goat Anti-rabbit IgG secondary antibody (green). Positive staining was localized to Nucleus and Cytoplasm.](http://images.novusbio.com/fullsize/YB1-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-98908-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: YB1 Antibody (4C7) [H00004904-M02] - Analysis of monoclonal antibody to YBX1 on HeLa cell . Antibody concentration 10 ug/ml.](http://images.novusbio.com/fullsize/YB1-Antibody-4C7-Immunocytochemistry-Immunofluorescence-H00004904-M02-img0003.jpg "Immunocytochemistry/Immunofluorescence: YB1 Antibody (4C7) [H00004904-M02] - Analysis of monoclonal antibody to YBX1 on HeLa cell . Antibody concentration 10 ug/ml.")
![ELISA: YB1 Antibody (4C7) [H00004904-M02] - Detection limit for recombinant GST tagged YBX1 is approximately 0.1ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/YB1-Antibody-4C7-ELISA-H00004904-M02-img0005.jpg)
![Immunocytochemistry/Immunofluorescence: YB1 Antibody (4F12) [H00004904-M01] - Analysis of monoclonal antibody to YBX1 on HeLa cell. Antibody concentration 20 ug/ml.](http://images.novusbio.com/fullsize/YB1-Antibody-4F12-Immunocytochemistry-Immunofluorescence-H00004904-M01-img0004.jpg "Immunocytochemistry/Immunofluorescence: YB1 Antibody (4F12) [H00004904-M01] - Analysis of monoclonal antibody to YBX1 on HeLa cell. Antibody concentration 20 ug/ml.")
![ELISA: YB1 Antibody (4F12) [H00004904-M01] - Detection limit for recombinant GST tagged YBX1 is approximately 0.3ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/YB1-Antibody-4F12-ELISA-H00004904-M01-img0006.jpg)
![Immunohistochemistry-Paraffin: YB1 Antibody (YBX1/2430) [NBP3-07452] - Formalin-fixed, paraffin-embedded human Prostate Carcinoma stained with YB1 Mouse Monoclonal Antibody (YB1/2430)](http://images.novusbio.com/fullsize/YB1-Antibody-YBX1-2430-Immunohistochemistry-Paraffin-NBP3-07452-img0002.jpg "Immunohistochemistry-Paraffin: YB1 Antibody (YBX1/2430) [NBP3-07452] - Formalin-fixed, paraffin-embedded human Prostate Carcinoma stained with YB1 Mouse Monoclonal Antibody (YB1/2430)")
![Protein Array: YB1 Antibody (YBX1/2430) [NBP3-07452] - Analysis of Protein Array containing more than 19,000 full-length human proteins using YB1 Mouse Monoclonal Antibody (YB1/2430).](http://images.novusbio.com/fullsize/YB1-Antibody-YBX1-2430-Protein-Array-NBP3-07452-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: YB1 Antibody [NBP2-58086] - Staining of human cell line A-431 shows localization to nuclear membrane, cytosol & vesicles.](http://images.novusbio.com/fullsize/YB1-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-58086-img0001.jpg "Immunocytochemistry/Immunofluorescence: YB1 Antibody [NBP2-58086] - Staining of human cell line A-431 shows localization to nuclear membrane, cytosol & vesicles.")