XRCC3 Antibody (10F1/6) [HRP]

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Product Details

Summary
Reactivity Hu, Mu(-), Rt, Bv, Pm, ShSpecies Glossary
Applications WB, IHC, IHC-P
Clone
10F1/6
Clonality
Monoclonal
Host
Mouse
Conjugate
HRP

XRCC3 Antibody (10F1/6) [HRP] Summary

Immunogen
His-tagged human XRCC3, over-expressed in E. coli [UniProt# O43542]
Localization
Nuclear
Specificity
This is specific for XRCC3. NB100-180 does not cross-react with Rad51B, Rad51C, Rad51D, Rad51, or XRCC2 in Western analysis.
Predicted Species
Rat (93%), Primate (100%), Bovine (93%), Sheep (93%). Backed by our 100% Guarantee.
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
XRCC3
Purity
Protein A purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
Application Notes
This XRCC3 (10F1/6) antibody is useful for Immunocytochemistry/Immunofluorescence, Immunohistochemistry on paraffin-embedded sections and Western blot. In WB, a band can be seen at ~38 kDa.

The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Theoretical MW
38 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Readout System

Reactivity Notes

Human. Does not work in mouse. Predicted to react with monkey based on 100% sequence homology. Immunogen has 93% homology to sheep, rat, and bovine.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
PBS
Preservative
No Preservative
Purity
Protein A purified

Notes

This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.

Alternate Names for XRCC3 Antibody (10F1/6) [HRP]

  • CMM6
  • DNA repair protein XRCC3
  • X-ray repair complementing defective repair in Chinese hamster cells 3
  • X-ray repair cross-complementing protein 3

Background

Five human RAD51 homologs have been identified: XRCC2, XRCC3, RAD51B, RAD51C, and RAD51D. Each of these homologs interacts with one or more of the others, with all of the proteins involved in one complex or multiple smaller complexes. XRCC3 (x-ray cross complementing) was first identified as a human gene complementing the DNA damage sensitivity, chromosonal instability and impaired growth of the mutant hamster cell line irs1SF. This fact coupled with XRCC3 being a member of the RAD51 gene family have implied a role in the repair of DNA damage by homologous recombination.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol

FAQs for XRCC3 Antibody (NB100-180H) (0)

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Secondary Antibodies

 

Isotype Controls

Other Available Formats

Biotin Labeled NB100-180B
DyLight 350 Labeled NB100-180UV
DyLight 405 Labeled NB100-180V
DyLight 488 Labeled NB100-180G
DyLight 550 Labeled NB100-180R
DyLight 650 Labeled NB100-180C
DyLight 680 Labeled NB100-180FR
DyLight 755 Labeled NB100-180IR
FITC Labeled NB100-180F

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Contact Information

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Bioinformatics

Gene Symbol XRCC3