XPB Antibody

Western blot shows lysates of Daudi human Burkitt's lymphoma cell line, HeLa human cervical epithelial carcinoma cell line, MOLT‑4 human acute lymphoblastic leukemia cell line, NRK rat normal kidney cell line, and RAW 264.7 mouse monocyte/macrophage cell line. PVDF Membrane was probed with 1 µg/mL of Goat Anti-Human/Mouse/Rat XPB Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6349) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for XPB at approximately 86 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

• Reactivity: Hu, Mu, Rt
• Applications: WB
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

XPB Antibody Summary

• Immunogen: E. coli-derived recombinant human XPB
  Met1-Lys375
  Accession # P19447
• Specificity: Detects human, mouse, and rat XPB in Western blots.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: ERCC3
• Purity: Immunogen affinity purified
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: Immunogen affinity purified
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for XPB Antibody

• Basic transcription factor 2 89 kDa subunit
• BTF2 p89
• BTF2
• DNA excision repair protein ERCC-3
• DNA repair protein complementing XP-B cells
• EC 3.6.1
• EC 3.6.4.12
• ERCC3
• excision repair cross-complementing rodent repair deficiency, complementationgroup 3 (xeroderma pigmentosum group B complementing)
• GTF2H
• RAD25
• TFIIH basal transcription factor complex 89 kDa subunit
• TFIIH basal transcription factor complex helicase XPB subunit
• TFIIH p89
• TFIIH
• Xeroderma pigmentosum group B-complementing protein
• xeroderma pigmentosum, complementation group B
• XPB
• XPBC
• XPBTFIIH 89 kDa subunit

Background

XPB (Xeroderma pigmentosum group B complementing protein; also ERCC3 and TFIIH 89 kDa subunit) is an 86 kDa, nuclear member of the RAD25/XPB subfamily, helicase family of proteins. It is expressed in multiple cell types, including neurons, fibroblasts and keratinocytes. XPB is part of the 10 subunit TFIIH complex. As such, it appears to participate in both nucleotide excision repair (NER) and transcription initiation from RNA polymerase II promoters. During NER, XPB utilizes an intrinsic ATPase activity to resolve nucleotide excision and repair. Human XPB is 782 amino acids (aa) in length. It contains an NLS (aa 6‑18), multiple acidic-rich regions, an ATPase region (aa 327‑488) and one helicase domain (aa 542‑702). Over aa 1‑375, human XPB shares 93% aa identity with mouse XPB.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for XPB Antibody (AF6349)(1)

Publication using AF6349

• Renaud S, Chakraborty D, Mason C, Rumi M, Vivian J, Soares M OVO-like 1 regulates progenitor cell fate in human trophoblast development. Proc Natl Acad Sci U S A, 2015;112(45):E6175-84. 2015 [PMID: 26504231]

Bioinformatics

• Gene Symbol: ERCC3
• Uniprot:
  • P19447()