XPB Antibody [Alexa Fluor® 488]

• Applications: WB
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Alexa Fluor 488

XPB Antibody [Alexa Fluor® 488] Summary

• Immunogen: E. coli-derived recombinant human XPB
  Met1-Lys375
  Accession # P19447
• Specificity: Detects human, mouse, and rat XPB in Western blots.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Purity Statement: Antigen Affinity-purified

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Background

XPB (Xeroderma pigmentosum group B complementing protein; also ERCC3 and TFIIH 89 kDa subunit) is an 86 kDa, nuclear member of the RAD25/XPB subfamily, helicase family of proteins. It is expressed in multiple cell types, including neurons, fibroblasts and keratinocytes. XPB is part of the 10 subunit TFIIH complex. As such, it appears to participate in both nucleotide excision repair (NER) and transcription initiation from RNA polymerase II promoters. During NER, XPB utilizes an intrinsic ATPase activity to resolve nucleotide excision and repair. Human XPB is 782 amino acids (aa) in length. It contains an NLS (aa 6‑18), multiple acidic-rich regions, an ATPase region (aa 327‑488) and one helicase domain (aa 542‑702). Over aa 1‑375, human XPB shares 93% aa identity with mouse XPB.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.