XLF Antibody - BSA Free

Western Blot: XLF Antibody [NBP1-31288] - Sample (30 ug of whole cell lysate) A: Hep G2 12% SDS PAGE; antibody diluted at 1:1000.

Immunohistochemistry-Paraffin: XLF Antibody [NBP1-31288] - Ovarian carcinoma.

Immunoprecipitation: XLF Antibody [NBP1-31288] - IP samples: Jurkat whole cell extract A. Control with 3 ug of preimmune Rabbit IgG B. Immunoprecipitation of XLF protein by 3 ug XLF antibody [N3C3]

XLF antibody [N3C3] detects XLF protein at nucleus by immunofluorescent analysis.Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: XLF protein stained by XLF antibody [N3C3] (NBP1-31288) diluted at 1:1000.Blue: Hoechst 33342 staining.

• Reactivity: Hu, Pm
• Applications: WB, ICC/IF, IHC, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

XLF Antibody - BSA Free Summary

• Immunogen: Recombinant protein encompassing a sequence within the center region of human XLF. The exact sequence is proprietary.
• Localization: Nucleus
• Predicted Species: Monkey (93%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: NHEJ1
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 10 - 1:500
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Immunoprecipitation 1:100-1:500
  • Western Blot 1:500-1:3000

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS, 20% Glycerol
• Preservative: 0.01% Thimerosal
• Purity: Antigen Affinity-purified

Alternate Names for XLF Antibody - BSA Free

• Cernunnos
• non-homologous end-joining factor 1
• nonhomologous end-joining factor 1
• Protein cernunnos
• XLFFLJ12610
• XRCC4-like factor

Background

Double-strand breaks in DNA result from genotoxic stresses and are among the most damaging of DNA lesions. This gene encodes a DNA repair factor essential for the nonhomologous end-joining pathway, which preferentially mediates repair of double-stranded breaks. Mutations in this gene cause different kinds of severe combined immunodeficiency disorders.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Bioinformatics

• Gene Symbol: NHEJ1