XLF Antibody

Images

 
Western Blot: XLF Antibody [NBP1-31288] - Sample (30 ug of whole cell lysate) A: Hep G2 12% SDS PAGE; antibody diluted at 1:1000.
Immunocytochemistry/ Immunofluorescence: XLF Antibody [NBP1-31288] - HeLa cells.
Immunohistochemistry-Paraffin: XLF Antibody [NBP1-31288] - Ovarian carcinoma.
Immunoprecipitation: XLF Antibody [NBP1-31288] - IP samples: Jurkat whole cell extract A. Control with 3 ug of preimmune Rabbit IgG B. Immunoprecipitation of XLF protein by 3 ug XLF antibody [N3C3]

Product Details

Summary
Reactivity Hu, MkSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1 mg/ml

Order Details

XLF Antibody Summary

Immunogen
Recombinant protein encompassing a sequence within the center region of human XLF. The exact sequence is proprietary.
Localization
Nucleus
Predicted Species
Monkey (93%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
NHEJ1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:3000
  • Immunocytochemistry/Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 10 - 1:500
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Immunoprecipitation 1:100-1:500

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.0), 1.0% BSA and 20% Glycerol
Preservative
0.01% Thimerosal
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for XLF Antibody

  • Cernunnos
  • non-homologous end-joining factor 1
  • nonhomologous end-joining factor 1
  • Protein cernunnos
  • XLFFLJ12610
  • XRCC4-like factor

Background

Double-strand breaks in DNA result from genotoxic stresses and are among the most damaging of DNA lesions. This gene encodes a DNA repair factor essential for the nonhomologous end-joining pathway, which preferentially mediates repair of double-stranded breaks. Mutations in this gene cause different kinds of severe combined immunodeficiency disorders.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for XLF Antibody (NBP1-31288) (0)

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Secondary Antibodies

 

Isotype Controls

Additional XLF Products

Bioinformatics Tool for XLF Antibody (NBP1-31288)

Discover related pathways, diseases and genes to XLF Antibody (NBP1-31288). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
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Diseases for XLF Antibody (NBP1-31288)

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Pathways for XLF Antibody (NBP1-31288)

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PTMs for XLF Antibody (NBP1-31288)

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Research Areas for XLF Antibody (NBP1-31288)

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Blogs on XLF.

Ku70: The DNA's Mr. Fix-it
Ku70, known by several synonyms including X-ray repair cross-complementing, 5'-deoxyribose-5-phosphate lyase Ku70 protein 6, 70 kDa subunit of Ku antigen, XRCC6, and G22P1, is a 70 kDa protein that was shown to be involved in multiple cellular pathway...  Read full blog post.

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Calculators

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Bioinformatics

Gene Symbol NHEJ1