Xg Antibody [Unconjugated]

Human blood erythrocytes were stained with Goat Anti-Human Xg Antigen Affinity‑purified Polyclonal Antibody (Catalog # AF3699, filled histogram) or isotype control antibody (Catalog # AB-108-C, open histogram), followed by Phycoerythrin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0107).

• Reactivity: Hu
• Applications: WB, Flow, CyTOF-ready
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

Xg Antibody [Unconjugated] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant human Xg
  Gln22-Lys142
  Accession # P55808
• Specificity: Detects human Xg in direct ELISAs and Western blots.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: XG
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Flow Cytometry 0.25 ug/10^6 cells
  • Western Blot 0.1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Xg Antibody [Unconjugated]

• glycoprotein Xg
• MGC118758
• MGC118759
• MGC118760
• MGC118761
• PBDX
• Protein PBDX
• Xg blood group (pseudoautosomal boundary-divided on the X chromosome)
• Xg blood group
• XG glycoprotein
• Xg
• XGPY

Background

Xg, also known as PBDX (pseudoautosomal boundary divided on the X chromosome), is a type I transmembrane glycoprotein that belongs to the CD99 family. It is an erythrocyte membrane protein that is used as the basis for the Xg blood group system. Mature Xg is a 26 kDa glycoprotein that consists of a 121 aa extracellular domain, a transmembrane segment, and a 17 aa cytoplasmic tail. Xg isoforms with either a 15 aa insertion or a deletion of nearly the entire ECD have been reported. Within the extracellular domain, human Xg shares 100% aa sequence identity with mouse Xg.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: XG
• Uniprot:
  • P55808()