WWP1 Antibody (1A7)

Images

 
Western Blot: WWP1 Antibody (1A7) [H00011059-M01] - Analysis of WWP1 expression in transfected 293T cell line by WWP1 monoclonal antibody (M01A), clone 1A7.Lane 1: WWP1 transfected lysate(105.2 KDa).Lane 2: ...read more
Immunocytochemistry/ Immunofluorescence: WWP1 Antibody (1A7) [H00011059-M01] - Analysis of monoclonal antibody to WWP1 on A-431 cell. Antibody concentration 10 ug/ml.
Immunohistochemistry-Paraffin: WWP1 Antibody (1A7) [H00011059-M01] - Analysis of monoclonal antibody to WWP1 on formalin-fixed paraffin-embedded human stomach. Antibody concentration 3 ug/ml.
Western Blot: WWP1 Antibody (1A7) [H00011059-M01] - WWP1 expression in A-431 ( Cat # L015V1 ).
Immunoprecipitation: WWP1 Antibody (1A7) [H00011059-M01] - Analysis of WWP1 transfected lysate using anti-WWP1 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with WWP1 MaxPab rabbit polyclonal ...read more
ELISA: WWP1 Antibody (1A7) [H00011059-M01] - Detection limit for recombinant GST tagged WWP1 is 0.03 ng/ml as a capture antibody.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA, ICC/IF, IHC, IHC-P, IP
Clone
1A7
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

WWP1 Antibody (1A7) Summary

Immunogen
WWP1 (NP_008944, 152 a.a. ~ 261 a.a) partial recombinant protein with GST tag.CSSSPTIEIQENGDALHENGEPSARTTARLAVEGTNGIDNHVPTSTLVQNSCCSYVVNGDNTPSSPSQVAARPKNTPAPKPLASEPADDTVNGESSSFAPTDNASVTGT
Specificity
WWP1 - WW domain containing E3 ubiquitin protein ligase 1 (1A7)
Isotype
IgG2a Kappa
Clonality
Monoclonal
Host
Mouse
Gene
WWP1
Purity
Ascites
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500
  • ELISA
  • Immunocytochemistry/Immunofluorescence 10ug/mL
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 3ug/mL
  • Immunoprecipitation
Application Notes
Antibody reactive against cell lysate and recombinant protein for Western Blot. Has also been used for immunofluoresence, immunohistochemistry (paraffin), and ELISA.
Publications
Read Publications using
H00011059-M01 in the following applications:

  • 1 publication
  • WB
    4 publications

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.4)
Preservative
No Preservative
Purity
Ascites

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for WWP1 Antibody (1A7)

  • AIP5
  • AIP5Nedd-4-like ubiquitin-protein ligase
  • atrophin-1 interacting protein 5
  • Atrophin-1-interacting protein 5
  • DKFZP434D2111
  • EC 6.3.2
  • EC 6.3.2.-
  • HSDRP1
  • NEDD4-like E3 ubiquitin-protein ligase WWP1
  • TGIF-interacting ubiquitin ligase 1
  • TIUL1
  • Tiul11
  • WW domain containing E3 ubiquitin protein ligase 1
  • WW domain-containing protein 1
  • WWP1

Background

WW domain-containing proteins are found in all eukaryotes and play an important role in the regulation of a wide variety of cellular functions such as protein degradation, transcription, and RNA splicing. This gene encodes a protein which contains 4 tandem WW domains and a HECT (homologous to the E6-associated protein carboxyl terminus) domain. The encoded protein belongs to a family of NEDD4-like proteins, which are E3 ubiquitin-ligase molecules and regulate key trafficking decisions, including targeting of proteins to proteosomes or lysosomes. Alternative splicing of this gene generates at least 6 transcript variants; however, the full length nature of these transcripts has not been defined.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for WWP1 Antibody (H00011059-M01)(13)

We have publications tested in 2 confirmed species: Human, Primate.

We have publications tested in 2 applications: IHC-P, WB.


Filter By Application
IHC-P
(1)
WB
(4)
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Filter By Species
Human
(2)
Primate
(1)
All Species
Showing Publications 1 - 10 of 13. Show All 13 Publications.
Publications using H00011059-M01 Applications Species
Qin J, Zhou Z, Chen W et al. BAP1 promotes breast cancer cell proliferation and metastasis by deubiquitinating KLF5. Nat Commun 2015 [PMID: 26419610] (WB) WB
Basheer WA, Harris BS, Mentrup HL et al. Cardiomyocyte-specific overexpression of the ubiquitin ligase Wwp1 contributes to reduction in connexin 43 and arrhythmogenesis. J Mol Cell Cardiol. 2015 Sep 16 [PMID: 26386426]
Courivaud T, Ferrand N, Elkhattouti A et al. Functional Characterization of a WWP1/Tiul1 Tumor-derived Mutant Reveals a Paradigm of Its Constitutive Activation in Human Cancer. J Biol Chem. 2015 Jul 07 [PMID: 26152726]
Ge F, Chen W, Qin J et al. Ataxin-3 like (ATXN3L), a member of the Josephin family of deubiquitinating enzymes, promotes breast cancer proliferation by deubiquitinating Kruppel-like factor 5 (KLF5). Oncotarget 2015 Aug 28 [PMID: 26079537]
Chaudhary N, Maddika S. WWP2-WWP1 Ubiquitin Ligase Complex Coordinated by PPM1G Maintains the Balance Between Cellular p73 and deltaNp73 Levels. Mol Cell Biol. 2014 Jul 28 [PMID: 25071155]
Cheng Q, Cao X, Yuan F et al. Knockdown of WWP1 inhibits growth and induces apoptosis in hepatoma carcinoma cells through the activation of caspase3 and p53. Biochem Biophys Res Commun. 2014 May 01 [PMID: 24792179]
Yeung B, Ho KC, Yang X. WWP1 E3 Ligase Targets LATS1 for Ubiquitin-Mediated Degradation in Breast Cancer Cells PLoS One 2013 [PMID: 23573293] (WB, Primate, Human) WB Primate, Human
Shu L, Zhang H, Boyce BF, Xing L. Ubiquitin E3 ligase Wwp1 negatively regulates osteoblast function by inhibiting osteoblast differentiation and migration. J Bone Miner Res. 2013 Sep [PMID: 23553732] (WB) WB
Cao X, Xue L, Han L et al. WWP1 delays cellular senescence by promoting p27Kip1 degradation in human diploid fibroblasts. J Biol Chem. 2011 Jul 27 [PMID: 21795702]
Peschiaroli A, Scialpi F, Bernassola F et al. The E3 ubiquitin ligase WWP1 regulates DeltaNp63-dependent transcription through Lys63 linkages. Biochem Biophys Res Commun 402(2):425-30. 2010 Nov 12. [PMID: 20951678]
Show All 13 Publications.

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Product General Protocols

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Video Protocols

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ICC/IF Video Protocol

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Bioinformatics

Gene Symbol WWP1
Entrez
OMIM