WWOX Antibody [Alexa Fluor® 350]

• Reactivity: Hu, Mu, Rt
• Applications: WB
• Clonality: Polyclonal
• Host: Sheep
• Conjugate: Alexa Fluor 350

WWOX Antibody [Alexa Fluor® 350] Summary

• Immunogen: E. coli-derived recombinant human WWOX
  Asn36-Val126
  Accession # Q9NZC7
• Specificity: Detects human, mouse, and rat WWOX in Western blots.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Sheep
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Western Blot

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for WWOX Antibody [Alexa Fluor® 350]

• D16S432E
• EC 1.1.1
• EC 1.1.1.-
• FOR
• FORHHCMA56
• FRA16D
• Fragile site FRA16D oxidoreductase
• SDR41C1
• short chain dehydrogenase/reductase family 41C, member 1
• WOX1
• WOX1PRO0128
• WW domain containing oxidoreductase
• WW domain-containing oxidoreductase
• WW domain-containing protein WWOX
• WWOX

Background

WWOX (WW domain-containing oxidoreductase; also WOX1 and FOR) is a 46 kDa cytoplasmic member of the short-chain dehydrogenases/reductases (or SDR) family of molecules. It is expressed in divergent cell types such as breast epithelium, keratinocytes, osteoblasts, and osteoclasts. WWOX has multiple effects, one of which is that of tumor suppressor. Here, WWOX is likely activated by either TGF-beta binding to surface hyaluronoglucosaminidase 2, or C1q binding to C1qR, which, in both cases, initiates cell apoptosis. Human WWOX is 414 amino acids (aa) in length and contains two WW (TrpTrp) domains (aa 16‑90) with an intervening NLS (aa 50‑55), plus an SDR region that contains a mitochondrial targeting sequence (aa 121‑330). There are multiple phosphorylation sites and multiple splice variants that impact intracellular localization. One shows a deletion of aa 173‑352, a second shows a Lys substitution for aa 36‑414, a third contains a 17 aa substitution for
aa 173‑414, a fourth possesses a 76 aa substitution for aa 138‑414, a fifth shows an 11 aa substitution for aa 353‑414, while a sixth contains a 175 aa substitution for aa 137‑414. Over aa 36‑126, human and mouse WWOX are identical in aa sequence.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.