WT1 Antibody

Product Discontinued

WT1 Antibody Summary

• Immunogen: Synthetic peptide (Human) (C terminal).
• Localization: Cytoplasmic and Nuclear
• Specificity: Wilms Tumor
• Clonality: Polyclonal
• Host: Rabbit
• Gene: WT1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin Neat
• Application Notes: IHC-P: Use neat for 10 min at RT. Boil tissue sections in 10mM Citrate buffer, pH 6.0 for 10 min followed by cooling at RT for 20 min.Not tested in other applications.Optimal dilutions/concentrations should be determined by the end user.

Reactivity Notes

Cross-reacts with Human, Mouse and Rat.Not yet tested in other species.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 10mM PBS (pH 7.4) and BSA
• Preservative: Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for WT1 Antibody

• AWT1
• GUD
• NPHS4GUD
• WAGR
• Wilms tumor 1
• Wilms tumor protein
• WIT-2
• WT1
• WT33

Background

WT1 encodes multiple isoforms of a zinc finger transcription factor essential for correct mammalian urogenital development. Different WT1 isoforms are thought to regulate transcription and participate in mRNA processing, functions reflected by a complex sub-nuclear distribution. In humans, the preservation of the correct isoform ratio and their localization has been reported to be vital, and its disruption to result in disease. This gene has been reported as a useful marker for several types of illnesses. Mutations in this gene lead to the development of Wilm's Tumor, a sporadic and familial childhood kidney tumor.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: WT1
• Entrez:
  • 7490(Human)
  • 24883(Rat)