WT1 Antibody (960525) [Alexa Fluor® 488]

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications Flow
Clone
960525
Clonality
Monoclonal
Host
Mouse
Conjugate
Alexa Fluor 488

WT1 Antibody (960525) [Alexa Fluor® 488] Summary

Immunogen
E.coli-derived recombinant human WT1
Val195-Thr317
Accession # P19544
Specificity
Detects human WT1 in direct ELISAs.
Source
N/A
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Intracellular Staining by Flow Cytometry 0.25-1 ug/10^6 cells

Packaging, Storage & Formulations

Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.
Buffer
Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for WT1 Antibody (960525) [Alexa Fluor® 488]

  • AWT1
  • GUD
  • NPHS4GUD
  • WAGR
  • Wilms tumor 1
  • Wilms tumor protein
  • WIT-2
  • WT1
  • WT33

Background

WT1 (Wilms' tumor protein 1; also WT33) is a 52‑54 kDa, nuclear member of the EGR C2H2‑type zinc‑finger family of proteins. Although its predicted MW is 49 kDa, it runs anomalously in SDS‑PAGE, likely due to a high proline content. It is widely expressed, being found in developing Sertoli cells, glomerular podocytes, neurons, and CD34+ stem cells. Human WT1 is 449 amino acids (aa) in length. It contains a Pro‑rich domain (aa 27‑83) and four consecutive C2H2 zinc finger regions (aa 323‑347; 353‑377; 383‑405; 414‑438). WT1 forms homodimers, and interacts with multiple molecules. Interaction with the zinc fingers generally promotes gene transcription, while N‑terminal interactions block gene transcription. There are at least two dozen splice variants. Some are combinations of deletions of aa 250‑266 and 408‑410, plus an alternate start site 68 aa upstream of the standard site, and a three aa substitution for aa 1‑147. Over aa 127‑249, human WT1 shares 98% aa identity with mouse WT1.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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FAQs for WT1 Antibody (IC57291G) (0)

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Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 IC57291U
Alexa Fluor 405 IC57291V
Alexa Fluor 488 IC57291G
Alexa Fluor 594 IC57291T
Alexa Fluor 647 IC57291R
Alexa Fluor 700 IC57291N
Alexa Fluor 750 IC57291S

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Routine WT1 Antibody Screen Uncovers an Exciting New Cancer-Cleaning Enzyme
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Bioinformatics

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