WSTF Antibody

Western Blot: WSTF Antibody [NBP3-03399] - Analysis of extracts of various cell lines, using WSTF antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit.

Immunocytochemistry/Immunofluorescence: WSTF Antibody [NBP3-03399] - Analysis of HeLa cells using WSTF antibody at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Immunohistochemistry-Paraffin: WSTF Antibody [NBP3-03399] - Paraffin-embedded mouse heart using WSTF antibody at dilution of 1:100 (40x lens).

Immunohistochemistry-Paraffin: WSTF Antibody [NBP3-03399] - Paraffin-embedded rat brain using WSTF antibody at dilution of 1:100 (40x lens).

Immunohistochemistry-Paraffin: WSTF Antibody [NBP3-03399] - Paraffin-embedded mouse lung using WSTF antibody at dilution of 1:100 (40x lens).

Immunohistochemistry-Paraffin: WSTF Antibody [NBP3-03399] - Paraffin-embedded mouse brain using WSTF antibody at dilution of 1:100 (40x lens).

Immunohistochemistry-Paraffin: WSTF Antibody [NBP3-03399] - Paraffin-embedded mouse stomach using WSTF antibody at dilution of 1:100 (40x lens).

Knockout Validated: WSTF Antibody [NBP3-03399] - Analysis of extracts from normal (control) and BAZ1B knockout (KO) HeLa cells, using WSTF antibody at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.

• Reactivity: Hu, Mu, Rt
• Applications: WB, ICC/IF, IHC, IHC-P, KO
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated

WSTF Antibody Summary

• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-260 of human WSTF (NP_115784.1).
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: BAZ1B
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1:500-1:2000
  • Immunocytochemistry/Immunofluorescence 1:50-1:200
  • Immunohistochemistry 1:50-1:200
  • Immunohistochemistry-Paraffin
  • Knockout Validated
• Theoretical MW: 170 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS with 50% glycerol, pH7.3.
• Preservative: 0.02% Sodium Azide
• Purity: Affinity purified

Alternate Names for WSTF Antibody

• bromodomain adjacent to zinc finger domain, 1B
• williams-Beuren syndrome chromosomal region 10 protein

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: BAZ1B