WRN Antibody - BSA Free

Immunohistochemistry-Paraffin: WRN Antibody [NBP1-87143] - Staining of human placenta shows strong nuclear positivity in trophoblastic cells and moderate nuclear and cytoplasmic staining in decidua cells.

Immunohistochemistry-Paraffin: WRN Antibody [NBP1-87143] -Staining of human testis shows moderate nuclear positivity in Leydig cells.

Immunohistochemistry-Paraffin: WRN Antibody [NBP1-87143] -Staining of human rectum shows strong nuclear positivity in glandular cells.

Immunohistochemistry-Paraffin: WRN Antibody [NBP1-87143] - Staining of human liver shows no positivity in hepatocytes as expected.

• Reactivity: Hu, Mu
• Applications: IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

WRN Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit WRN Antibody - BSA Free (NBP1-87143) is a polyclonal antibody validated for use in IHC. Anti-WRN Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: This antibody was developed against Recombinant Protein corresponding to amino acids: KADIRQVIHYGAPKDMESYYQEIGRAGRDGLQSSCHVLWAPADINLNRHLLTEIRNEKFRLYKLKMMAKMEKYLHSSRCRRQIILSHFEDKQVQKASLGIMGTEKCCDNCRSRLDHCYSMDDSEDTSWDFGPQAFKLLSAVD
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: WRN
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:50 - 1:200
  • Immunohistochemistry-Paraffin 1:50 - 1:200
• Application Notes: For IHC-Paraffin, HIER pH 6 retrieval is recommended.
• Control Peptide:

  WRN Protein (NBP1-87143PEP)

Reactivity Notes

Mouse reactivity reported in (PMID: 32919863). Rat (82%)

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2) and 40% Glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Affinity purified

Alternate Names for WRN Antibody - BSA Free

• DKFZp686C2056
• DNA helicase, RecQ-like type 3
• EC 3.1
• EC 3.1.-.-
• EC 3.6.1
• EC 3.6.4.12
• Exonuclease WRN
• RecQ protein-like 2
• RecQ3
• RECQ3RECQL3
• RECQL2
• RECQL2Werner syndrome ATP-dependent helicase
• Werner syndrome
• Werner syndrome, RecQ helicase-like
• WRN

Background

WRN encodes a member of the RecQ subfamily and the DEAH (Asp-Glu-Ala-His) subfamily of DNA and RNA helicases. DNA helicases are involved in many aspects of DNA metabolism, including transcription, replication, recombination, and repair. This protein contains a nuclear localization signal in the C-terminus and shows a predominant nucleolar localization. It possesses an intrinsic 3' to 5' DNA helicase activity, and is also a 3' to 5' exonuclease. Based on interactions between this protein and Ku70/80 heterodimer in DNA end processing, this protein may be involved in the repair of double strand DNA breaks. Defects in this gene are the cause of Werner Syndrome, an autosomal recessive disorder characterized by premature aging.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for WRN Antibody (NBP1-87143)(1)

Publication using NBP1-87143

• Savva C, Sadiq M, Sheikh O et al. Werner syndrome protein expression in breast cancer Clin Breast Cancer 2020-09-13 [PMID: 32919863] (IF/IHC, Mouse)

Bioinformatics

• Gene Symbol: WRN