WRN Antibody (SR1313) - BSA Free

Western Blot: WRN Antibody (SR1313) [NBP3-22281] - Western blot analysis using NBP3-22281 in K562 cell lysate.

• Reactivity: Hu
• Applications: WB
• Clone: SR1313
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

WRN Antibody (SR1313) - BSA Free Summary

• Additional Information: Recombinant Monoclonal Antibody
• Immunogen: A synthesized peptide derived from human WRN (Uniprot #: Q14191)
• Specificity: Detects endogenous levels of total WRN
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: WRN
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1:500-1:2000
• Theoretical MW: 200 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS, pH 7.4, 150mM NaCl, 50% glycerol.
• Preservative: 0.02% Sodium Azide
• Purity: Affinity purified

Alternate Names for WRN Antibody (SR1313) - BSA Free

• DKFZp686C2056
• DNA helicase, RecQ-like type 3
• EC 3.1
• EC 3.1.-.-
• EC 3.6.1
• EC 3.6.4.12
• Exonuclease WRN
• RecQ protein-like 2
• RecQ3
• RECQ3RECQL3
• RECQL2
• RECQL2Werner syndrome ATP-dependent helicase
• Werner syndrome
• Werner syndrome, RecQ helicase-like
• WRN

Background

WRN encodes a member of the RecQ subfamily and the DEAH (Asp-Glu-Ala-His) subfamily of DNA and RNA helicases. DNA helicases are involved in many aspects of DNA metabolism, including transcription, replication, recombination, and repair. This protein contains a nuclear localization signal in the C-terminus and shows a predominant nucleolar localization. It possesses an intrinsic 3' to 5' DNA helicase activity, and is also a 3' to 5' exonuclease. Based on interactions between this protein and Ku70/80 heterodimer in DNA end processing, this protein may be involved in the repair of double strand DNA breaks. Defects in this gene are the cause of Werner Syndrome, an autosomal recessive disorder characterized by premature aging.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: WRN