Wnt-4 Antibody

Images

 
Western Blot: Wnt-4 Antibody [NBP2-20909] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with WNT4 antibody diluted at a dilution of 1:1000.
Immunohistochemistry-Paraffin: Wnt-4 Antibody [NBP2-20909] - Mouse kidney.
Western Blot: Wnt-4 Antibody [NBP2-20909] - Sample (30 ug of whole cell lysate) A: Non-transfected 293T lysates B: WNT4 transfected 293T lysates 10% SDS PAGE diluted at 1:5000
Western Blot: Wnt-4 Antibody [NBP2-20909] - Non-transfected (-) and Wnt4-transfected (+, including V5-tag) 293T whole cell extracts (100 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with WNT4 ...read more
Immunohistochemistry-Paraffin: Wnt-4 Antibody [NBP2-20909] - Analysis of paraffin-embedded U87 xenograft.

Product Details

Summary
Reactivity Hu, Mu, Rt, XpSpecies Glossary
Applications WB, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
0.78 mg/ml

Order Details

Wnt-4 Antibody Summary

Immunogen
Recombinant protein encompassing a sequence within the center region of human WNT4. The exact sequence is proprietary.
Localization
Secreted; extracellular space; extracellular matrix
Predicted Species
Xenopus (90%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
WNT4
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:10000
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
Theoretical MW
39 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Publications
Read Publication using NBP2-20909.

Reactivity Notes

Expected cross reactivity based on sequence homology: Japanese medaka (85%), Chicken (89%), Xenopus laevis (90%).

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
0.1M Tris (pH 7.0), 0.1M Glycine and 10% Glycerol
Preservative
0.01% Thimerosal
Concentration
0.78 mg/ml
Purity
Immunogen affinity purified

Alternate Names for Wnt-4 Antibody

  • protein Wnt-4
  • SERKAL
  • wingless-type MMTV integration site family, member 4
  • Wnt4
  • Wnt-4

Background

The WNT gene family consists of structurally related genes which encode secreted signaling proteins. These proteins have been implicated in oncogenesis and in several developmental processes, including regulation of cell fate and patterning during embryogenesis. This gene is a member of the WNT gene family, and is the first signaling molecule shown to influence the sex-determination cascade. It encodes a protein which shows 98% amino acid identity to the Wnt4 protein of mouse and rat. This gene and a nuclear receptor known to antagonize the testis-determining factor play a concerted role in both the control of female development and the prevention of testes formation. This gene and another two family members, WNT2 and WNT7B, may be associated with abnormal proliferation in breast tissue. Mutations in this gene can result in Rokitansky-Kuster-Hauser syndrome and in SERKAL syndrome. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Wnt-4 Antibody (NBP2-20909)(1)

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Bioinformatics

Gene Symbol WNT4
Entrez
Uniprot