After August 17, 2026, Novus Biologicals products and services will no longer be available on this website; you will access all products and services on rndsystems.com. Create your R&D Systems online account today.
HAF005). A specific band for Wnt-4 was detected at approximately 39 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_lightbox" target="_blank">
HAF005). A specific band was detected for Wnt‑4 at approximately 39 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1." class="big_lightbox" target="_blank">
VC005). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to plasma membrane. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents." class="big_lightbox" target="_blank">
DY990). Sheep Anti-Human Wnt-4 Antigen Affinity-purified Polyclonal Antibody (AF6076) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (DY998) followed by Substrate Solution (DY999) and stopping the enzymatic reaction with Stop Solution (DY994)." class="big_lightbox" target="_blank">
DY990). Sheep Anti-Human Wnt-4 Antigen Affinity-purified Polyclonal Antibody (AF6076) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (DY998) followed by Substrate Solution (DY999) and stopping the enzymatic reaction with Stop Solution (DY994)." class="big_lightbox" target="_blank">
The WNT gene family consists of structurally related genes which encode secreted signaling proteins. These proteins have been implicated in oncogenesis and in several developmental processes, including regulation of cell fate and patterning during embryogenesis. WNT4 is a member of the WNT gene family, and is the first signaling molecule shown to influence the sex-determination cascade. It encodes a protein which shows 98% amino acid identity to the Wnt4 protein of mouse and rat. This gene and DAX1, a gene known to antagonize the testis-determining factor, play a concerted role in both the control of female development and the prevention of testes formation. WNT4 and another two family members, WNT2 and WNT7B, may be associated with abnormal proliferation in breast tissue.
![Western Blot Wnt-4 Antibody (55025) [Unconjugated]](http://images.novusbio.com/images/antibody/Wnt-4_MAB4751_Western_Blot_6205.jpg)
![Immunohistochemistry Wnt-4 Antibody (55010) [Unconjugated]](http://images.novusbio.com/images/antibody/Wnt-4_MAB475_Immunohistochemistry_12046.jpg)
![Immunocytochemistry Wnt-4 Antibody (55025) [Unconjugated]](http://images.novusbio.com/images/antibody/Wnt4_MAB4751_Immunocytochemistry__Immunofluorescence_20217.jpg)
![Western Blot Wnt-4 Antibody (55025) [Unconjugated]](http://images.novusbio.com/images/mab4751_human-mouse-wnt-4-mab-clone-55025-western-blot-2042022142455.jpg)
![Immunohistochemistry Wnt-4 Antibody (55025) [Unconjugated]](http://images.novusbio.com/images/antibody/Wnt4_MAB4751_Immunohistochemistry_20305.jpg)
![Immunohistochemistry Wnt-4 Antibody (55025) [Unconjugated]](http://images.novusbio.com/images/antibody/Wnt4_MAB4751_Immunohistochemistry_22697.jpg)
![ELISA Wnt-4 Antibody (55025) [Unconjugated]](http://images.novusbio.com/images/mab4751_human-mouse-wnt-4-mab-clone-55025-elisa-254202213243.jpg)
![ELISA Wnt-4 Antibody (55010) [Unconjugated]](http://images.novusbio.com/images/mab475_mouse-wnt-4-mab-clone-55010-elisa-2542022131110.jpg)
