Wnt-4 Antibody [Unconjugated]

Recombinant Human Wnt-4 protein was serially diluted 2-fold and captured by Rat Anti-Human/Mouse Wnt-4 Monoclonal Antibody (MAB475) coated on a Clear Polystyrene Microplate (DY990). Sheep Anti-Human Wnt-4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6076) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (DY998) followed by Substrate Solution (DY999) and stopping the enzymatic reaction with Stop Solution (DY994).

Recombinant Human Wnt-4 protein was serially diluted 2-fold and captured by Rat Anti-Human/Mouse Wnt-4 Monoclonal Antibody (MAB4751 coated on a Clear Polystyrene Microplate (DY990). Sheep Anti-Human Wnt-4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6076) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (DY998) followed by Substrate Solution (DY999) and stopping the enzymatic reaction with Stop Solution (DY994).

• Reactivity: Hu
• Applications: ELISA
• Clonality: Polyclonal
• Host: Sheep
• Conjugate: Unconjugated
• Concentration: LYOPH

Wnt-4 Antibody [Unconjugated] Summary

• Immunogen: Chinese hamster ovary cell line CHO-derived recombinant human Wnt-4
  Ser23-Arg351
  Accession # P56705
• Specificity: Detects human Wnt-4 in direct ELISAs. In direct ELISAs, approximately 10-15% cross-reactivity with recombinant human Wnt-3A and recombinant mouse Wnt-10B is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Sheep
• Gene: WNT4
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • ELISA

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Sterile PBS to a final concentration of 0.2 mg/mL.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Wnt-4 Antibody [Unconjugated]

• protein Wnt-4
• SERKAL
• wingless-type MMTV integration site family, member 4
• Wnt4
• Wnt-4

Background

Wnt-4 is a 38‑42 kDa member of the Wnt family of secreted glycoproteins, which act as short-range signaling molecules via Frizzled receptors and a cascade of intracellular signals in vertebrate embryogenesis (1‑2). Human Wnt-4 is synthesized as a 351 amino acid (aa) precursor with a 22 aa signal sequence and a 329 aa mature chain. The mature chain contains two potential sites for N-linked glycosylation. Relative to other members of the Wnt family, Wnt-4 contains 83 conserved aa, including 21 cysteines (1). Mature human Wnt-4 shares 99%, 98% and 99% aa sequence identity with mature mouse, rat and canine Wnt-4, respectively. Wnt-4 has been shown to play a critical role in the development of the reproductive system and in the formation of the kidneys, adrenals, pituitary gland, and mammary tissues (3‑6). In the development of the reproductive system, Wnt-4 expression is down‑regulated in the developing gonad after E11.5, although it persists in the developing ovary (2, 6). Targeted deletion of Wnt-4 results in masculinization of XX mice, with rudimentary development of the masculine internal (Wolffian) ducts and degeneration of the female (Mullerian) reproductive tract (2, 6). In addition to its involvement in urogenital development, Wnt-4 is also expressed in the perichondrium of the long bones (7), and promotes osteoblast differentiation (8). Wnt-4 may also be associated with abnormal proliferation in human breast tissue (9). In humans, mutations in Wnt-4 are the cause of SERKAL syndrome, a syndrome consisting of female to male sex reversal, renal, adrenal, and lung dysgenesis, and developmental defects (3), and Rokitansky-Kuster-Hauser syndrome, which is characterized by utero-vaginal atresia in otherwise phenotypically normal females with normal 46, XX karyotype (10).

1. Gavin, B.J. et al. (1990) Genes Dev. 4:2319.
2. Jordan, B.K. et al. (2001) Am. J. Hum. Genet. 68:1102.
3. Mandel, H. et al. (2008) Am. J. Hum. Genet. 82:39.
4. Bernard, P. and V.R. Harley (2007) Int. J. Biochem. Cell Biol. 39:31.
5. Kuulasmaa, T. et al. (2008) Horm. Metab. Res. 40:668.
6. Vainio, S. et al. (1999) Nature 397:405.
7. Gao, X. (2004) Genes Dev. 18:2404.
8. Chang, J. et al. (2007) J. Biol. Chem. 282:30938.
9. Huguet, E.L. et al. (1994) Cancer Res. 54:2615.
10. Philibert, P. et al. (2008) J. Clin. Endocrinol. Metab. 93:895.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Wnt-4 Antibody (AF6076)(1)

Publication using AF6076

• Tianzhi Huang, Angel A. Alvarez, Rajendra P. Pangeni, Craig M M. Horbinski, Songjian Lu, Sung-Hak Kim et al. A regulatory circuit of miR-125b/miR-20b and Wnt signalling controls glioblastoma phenotypes through FZD6-modulated pathways Nature Communications 2016-10-04 [PMID: 27698350]

Bioinformatics

• Gene Symbol: WNT4
• Uniprot:
  • P56705(Human)