Wnt-3a Antibody (1E6G4) - BSA Free

Western Blot: Wnt-3a Antibody (1E6G4) [NBP2-52588] - Analysis using WNT3A mouse mAb against A549 (1), HepG2 (2), and A431 (3) cell lysate.

Immunocytochemistry/Immunofluorescence: Wnt-3a Antibody (1E6G4) [NBP2-52588] - Analysis of HeLa cells using WNT3A mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin.

Immunohistochemistry-Paraffin: Wnt-3a Antibody (1E6G4) [NBP2-52588] - Analysis of endometrial cancer tissues, mouse mab with DAB staining.

Flow Cytometry: Wnt-3a Antibody (1E6G4) [NBP2-52588] - Analysis of HeLa cells using WNT3A mouse mAb (green) and negative control (red).

Western Blot: Wnt-3a Antibody (1E6G4) [NBP2-52588] - Analysis using WNT3A mAb against HEK293 (1) and WNT3A (AA: 170-352)-hIgGFc transfected HEK293 (2) cell lysate.

Western Blot: Wnt-3a Antibody (1E6G4) [NBP2-52588] - Analysis using WNT3A mAb against human WNT3A (AA: 170-352) recombinant protein. (Expected MW is 47.1 kDa)

Immunohistochemistry-Paraffin: Wnt-3a Antibody (1E6G4) [NBP2-52588] - Analysis of rectum cancer tissues using WNT3A mouse mAb with DAB staining.

ELISA: Wnt-3a Antibody (1E6G4) [NBP2-52588] - Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);

• Reactivity: Hu
• Applications: WB, ELISA, Flow, ICC/IF, IHC, CyTOF-ready
• Clone: 1E6G4
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

Wnt-3a Antibody (1E6G4) - BSA Free Summary

• Immunogen: Purified recombinant fragment of human Wnt-3a (AA: 170-352) expressed in E. Coli.
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: WNT3A
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • ELISA 1:10000
  • Flow Cytometry 1:200-1:400
  • Immunocytochemistry/ Immunofluorescence 1:200-1:1000
  • Immunohistochemistry 1:200-1:1000
  • Immunohistochemistry-Paraffin
  • Western Blot 1:500-1:2000
• Application Notes: This antibody is Cytof ready.
• Theoretical MW: 39.4 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein G purified

Alternate Names for Wnt-3a Antibody (1E6G4) - BSA Free

• MGC119418
• MGC119419
• MGC119420
• protein Wnt-3a
• wingless-type MMTV integration site family, member 3A
• Wnt3a
• Wnt-3a

Background

Wingless-type MMTV integration site family, member 3A (Wnt3a) is a member of the Wnt family. The Wnt gene family is representative of a growing class of multifunctioanl factors that play a role in both tumorigenesis and patterning events required for normal differentiation and development. Wnt3a is a ligand for members of the frizzled family of seven transmembrane receptors. Anti-human Wnt3a mAb, clone 3A6, is derived from hybridization of mouse F0 myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human Wnt3a protein.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: WNT3A