Wnt-3a Antibody (1E6G4)

Images

 
Western Blot: Wnt-3a Antibody (1E6G4) [NBP2-52588] - Analysis using WNT3A mouse mAb against A549 (1), HepG2 (2), and A431 (3) cell lysate.
Immunohistochemistry-Paraffin: Wnt-3a Antibody (1E6G4) [NBP2-52588] - Immunohistochemical aAWNT3A mouse mAb with DAB staining.
Flow Cytometry: Wnt-3a Antibody (1E6G4) [NBP2-52588] - Analysis of HeLa cells using WNT3A mouse mAb (green) and negative control (red).
Western Blot: Wnt-3a Antibody (1E6G4) [NBP2-52588] - Analysis using WNT3A mAb against HEK293 (1) and WNT3A (AA: 170-352)-hIgGFc transfected HEK293 (2) cell lysate.
Western Blot: Wnt-3a Antibody (1E6G4) [NBP2-52588] - Analysis using WNT3A mAb against human WNT3A (AA: 170-352) recombinant protein. (Expected MW is 47.1 kDa)
Immunohistochemistry-Paraffin: Wnt-3a Antibody (1E6G4) [NBP2-52588] - Analysis of paraffin-embedded rectum cancer tissues using WNT3A mouse mAb with DAB staining.
Immunofluorescence: Wnt-3a Antibody (1E6G4) [NBP2-52588] - Analysis of HeLa cells using WNT3A mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin.
ELISA: Wnt-3a Antibody (1E6G4) [NBP2-52588] - Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA, Flow, IHC, IHC-P, CyTOF-ready, ICC/IF
Clone
1E6G4
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

Wnt-3a Antibody (1E6G4) Summary

Immunogen
Purified recombinant fragment of human WNT3A (AA: 170-352) expressed in E. Coli.
Isotype
IgG2b
Clonality
Monoclonal
Host
Mouse
Gene
WNT3A
Purity
Protein A or G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:2000
  • ELISA 1:10000
  • Flow Cytometry 1:200-1:400
  • Immunohistochemistry 1:200-1:1000
  • Immunohistochemistry-Paraffin
  • CyTOF-ready
  • Immunocytochemistry 1:200-1:1000
  • Immunofluorescence
Theoretical MW
39.4 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.05% Sodium Azide
Purity
Protein A or G purified

Alternate Names for Wnt-3a Antibody (1E6G4)

  • MGC119418
  • MGC119419
  • MGC119420
  • protein Wnt-3a
  • wingless-type MMTV integration site family, member 3A
  • Wnt3a
  • Wnt-3a

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Video Protocols

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol WNT3A
Entrez