Wnt-2 Antibody (HL1999) - Azide and BSA Free

Western Blot: Wnt-2 Antibody (HL1999) - Azide and BSA Free [NBP3-25752] - Rat tissue extract (50 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with Wnt2 antibody [HL1999] (NBP3-25752) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Western Blot: Wnt-2 Antibody (HL1999) - Azide and BSA Free [NBP3-25752] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Wnt2 antibody [HL1999] (NBP3-25752) diluted at 1:15000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Western Blot: Wnt-2 Antibody (HL1999) - Azide and BSA Free [NBP3-25752] - Mouse tissue extract (50 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with Wnt2 antibody [HL1999] (NBP3-25752) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Western Blot: Wnt-2 Antibody (HL1999) - Azide and BSA Free [NBP3-25752] - Whole zebrafish extract (30 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with Wnt2 antibody [HL1999] (NBP3-25752) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Wnt-2 antibody [HL1999] detects Wnt-2 protein by immunofluorescent analysis.Sample: Mock and transfected 293T cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: Wnt-2 stained by Wnt-2 antibody [HL1999] (NBP3-25752) diluted at 1:500.Blue: Fluoroshield with DAPI .

Whole cell extract (30 ug) was separated by 12% SDS-PAGE, and the membrane was blotted with Wnt-2 antibody [HL1999] (NBP3-25752) diluted at 1:7500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

• Reactivity: Hu, Mu, Rt, Ze
• Applications: WB
• Clone: HL1999
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: Azide and BSA Free

Wnt-2 Antibody (HL1999) - Azide and BSA Free Summary

• Description: Novus Biologicals Rabbit Wnt-2 Antibody (HL1999) - Azide and BSA Free (NBP3-25752) is a recombinant monoclonal antibody validated for use in WB. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Additional Information: Recombinant Monoclonal Antibody
• Immunogen: Recombinant fragment of human Wnt-2.
• Source: HEK293
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: WNT2
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1:5000-1:20000

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: No Preservative
• Purity: Protein A purified

Alternate Names for Wnt-2 Antibody (HL1999) - Azide and BSA Free

• INT1L1
• INT1L1secreted growth factor
• Int-1-like protein 1
• Int-1-related protein
• IRP
• IRPprotein Wnt-2
• wingless-type MMTV integration site family member 2
• Wnt2
• Wnt-2

Background

The WNT2 gene is a member of the WNT gene family. The WNT gene family consists of structurally related genes which encode secreted signaling proteins. These proteins have been implicated in oncogenesis and in several developmental processes, including regulat

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: WNT2