Recombinant Human Wnt-1 Protein

SDS-Page: Recombinant Human Wnt-1 Protein [NBP1-99372] - Purity of recombinant Wnt-1 (2 ug) was analyzed by reduced SDS-PAGE electrophoresis.

Product Discontinued

Recombinant Human Wnt-1 Protein Summary

• Description: Recombinant bioactive protein containing 343 amino acids for Human Wnt-1
  
  

  Source:E. coli
  Amino Acid Sequence:(P04628)

• Preparation Method: Determined to be >98% pure by SDS-PAGE
• Details of Functionality: The ED50 was determined by its ability to enhance BMP-2 induced alkaline phosphatase production by murine ATDC5 cells. The expected ED50 for this effect is 1.5 - 2.5 ng/ml in the presence of 200 ng/ml of human BMP-2.
• Source: E. coli
• Protein/Peptide Type: Recombinant Protein
• Gene: WNT1
• Purity: >98%, by SDS-PAGE
• Endotoxin Note: <0.1 ng/ug

Applications/Dilutions

• Dilutions:
  • Bioactivity
  • SDS-Page
• Theoretical MW: 38.4 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20 to -80C. Avoid freeze-thaw cycles.
• Buffer: Sterile filtered and lyophilized with no additives
• Concentration: Lyoph
• Purity: >98%, by SDS-PAGE
• Reconstitution Instructions: Reconstitute in dH2O to a concentration of 0.1 - 1.0 mg/ml. The solution can then be diluted into other aqueous buffers and stored at 4C for 1 week or -20C for long-term storage.

Alternate Names for Recombinant Human Wnt-1 Protein

• Int-1
• INT1Proto-oncogene Int-1 homolog
• proto-oncogene Wnt-1
• wingless-type MMTV integration site family, member 1 (oncogene INT1)
• wingless-type MMTV integration site family, member 1
• Wnt1
• Wnt-1

Background

Wnt-1 is a secreted protein that signals through the Frizzled family of cell surface receptors and is required for normal embryonic development. Wnt-1 activation induces a complex signaling cascade that ultimately leads to the increased expression of over fifty genes. An important component of Wnt-1 signaling is the stabilization, and resulting accumulation, of the intraCellular signaling protein, Beta -catenine. Wnt signaling induces and maintains the transformed phenotype and, in certain embryonic cell lines, supports self renewal in the absence of significant differentiation. Elevated levels of Wnt proteins are associated with tumorigenesis and are present in numerous human breast cancers. Mature human Wnt-1 is a glycosylated protein containing 343 amino acid residues. Recombinant human Wnt-1 is a 38.4 kDa, non-glycosylated protein containing 343 amino acid residues.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

Bioinformatics

• Gene Symbol: WNT1