WNK4 Antibody Blocking Peptide

• Reactivity: Hu
• Applications: AC
• Concentration: 1.0 mg/ml

WNK4 Antibody Blocking Peptide Summary

• Description: A WNK4 antibody blocking peptide.
• Immunogen: A synthetic peptide made to a region of the human WNK4 protein between residues 200-300. [UniProt# Q96J92]
• Protein/Peptide Type: Antibody Blocking Peptide
• Gene: WNK4

Applications/Dilutions

• Dilutions:
  • Antibody Competition
• Application Notes: This peptide is useful as a blocking peptide for NB600-284. For further blocking peptide related protocol, click here.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS
• Concentration: 1.0 mg/ml

Alternate Names for WNK4 Antibody Blocking Peptide

• EC 2.7.11
• EC 2.7.11.1
• PHA2B
• PRKWNK4protein kinase, lysine deficient 4
• Protein kinase lysine-deficient 4
• Protein kinase with no lysine 4
• serine/threonine-protein kinase WNK4
• WNK lysine deficient protein kinase 4

Background

WNK4 (protein kinase with no lysine 4) is a novel serine/threonine kinase which plays critical role in regulation of electrolyte homeostasis, signal transduction, survival and proliferation. WNK4 is highly expressed in kidney, colon and skin where it forms a part of tight junction complex and interacts with/activate several proteins including SCNN1A, SCNN1B, SCNN1D, SGK1, TRPV5, NEDD4L and TRPV6. It control SLC12A3 (thiazide-sensitive Na-Cl cotransporter) activity by phosphorylation process that prevents membrane trafficking of SLC12A3. WNK4 inhibits KCNJ1 (renal K+ channel) via a kinase-independent action leading to induction of protein clearance from the cell surface by clathrin-dependent endocytosis. WNK4 acts as a molecular switch that controls NaCl reabsorption and K+ secretion for maintaining integrated homeostasis. Autophosphorylation of Ser-335 is essential for WNK4's activity whereas phosphorylation of Ser-331 also increase its activity. Mutations of WNK4 can cause pseudohypoaldosteronism type alpha (PHA alpha), an autosomal dominant disease characterized by hyperkalemia, metabolic acidosis and hypertension.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

Bioinformatics

• Gene Symbol: WNK4