WNK1 Antibody [FITC]

Product Discontinued

WNK1 Antibody [FITC] Summary

• Immunogen: A synthetic peptide within the C-terminal region of human WNK1 (within the last 350 amino acids). [UniProt# Q9H4A3]
• Localization: Cytoplasmic
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: WNK1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence
  • Western Blot
• Application Notes: This WNK1 antibody is useful for Immunocytochemistry/Immunofluorescence and Western blot. In WB, this antibody recognizes the short form of WNK1, ~190 kDa. The theoretical molecular weight of human WNK1 is ~251 kDa and rat WNK1 is ~230 kDa. In ICC/IF cytomplasmic staining was observed.
  
  The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Human, mouse and rat.

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark.
• Buffer: PBS
• Preservative: 0.05% Sodium Azide
• Purity: Immunogen affinity purified

Alternate Names for WNK1 Antibody [FITC]

• EC 2.7.11
• EC 2.7.11.1
• Erythrocyte 65 kDa protein
• HSAN2hereditary sensory neuropathy, type II
• HSN2MGC163339
• hWNK1
• KDP
• KDPMGC163341
• KIAA0344
• Kinase deficient protein
• p65
• PHA2C
• PRKWNK1
• PRKWNK1PSK
• prostate-derived sterile 20-like kinase
• Protein kinase lysine-deficient 1
• Protein kinase with no lysine 1
• protein kinase, lysine deficient 1
• serine/threonine-protein kinase WNK1
• WNK lysine deficient protein kinase 1
• WNK1

Background

WNK1 (with no-lysine kinase 1) is a serine-threonine kinase regulating many ion channels implicated in sodium/potassium transport and plays a key role in regulation of electrolyte homeostasis, cell signaling, survival, and proliferation. It is ubiquitously expressed in various tissues with higher levels in kidney and cardiovascular system, and acts as an important modulator of salt homeostasis, regulating the balance between renal sodium reabsorption and potassium excretion. WNK1 activates SCNN1A, SCNN1B, SCNN1D and SGK1, and controls sodium/chloride ion transport through WNK4 inhibition, by either phosphorylating the kinase or via an interaction between WNK4 and the autoinhibitory domain of WNK1. Increased WNK1 expression would have the net result of increasing NaCl reabsorption, leading to volume expansion-induced hypertension. WNK1 has also been suggested to play a role in actin cytoskeletal reorganization. Defects in WNK1 are a cause of pseudohypoaldosteronism type 2C (PHA2C), and hereditary sensory and autonomic neuropathy type 2A (HSAN2A).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: WNK1