WNK1 Antibody (JG36-91)

Western Blot: WNK1 Antibody (JG36-91) [NBP2-75712] - Analysis of WNK1 on A549 cell lysates using anti-WNK1 antibody at 1/1,000 dilution.

Immunohistochemistry-Paraffin: WNK1 Antibody (JG36-91) [NBP2-75712] - Analysis of paraffin-embedded rat kidney tissue using anti-WNK1 antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: WNK1 Antibody (JG36-91) [NBP2-75712] - Analysis of paraffin-embedded human skin tissue using anti-WNK1 antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: WNK1 Antibody (JG36-91) [NBP2-75712] - Analysis of paraffin-embedded human breast cancer tissue using anti-WNK1 antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: WNK1 Antibody (JG36-91) [NBP2-75712] - Analysis of paraffin-embedded mouse testis tissue using anti-WNK1 antibody. Counter stained with hematoxylin.

Product Discontinued

WNK1 Antibody (JG36-91) Summary

• Additional Information: Recombinant Monoclonal Antibody.
• Immunogen: Synthetic peptide within Human WNK1 aa 33-82 / 2382. (SwissProt: Q9H4A3 Human; SwissProt: P83741 Mouse; SwissProt: Q9JIH7 Rat)
• Localization: Cytoplasm.
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: WNK1
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:50-1:200
  • Western Blot 1:500-1:2000
• Theoretical MW: 251 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: TBS (pH7.4), 0.05% BSA, 40% Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for WNK1 Antibody (JG36-91)

• EC 2.7.11
• EC 2.7.11.1
• Erythrocyte 65 kDa protein
• HSAN2hereditary sensory neuropathy, type II
• HSN2MGC163339
• hWNK1
• KDP
• KDPMGC163341
• KIAA0344
• Kinase deficient protein
• p65
• PHA2C
• PRKWNK1
• PRKWNK1PSK
• prostate-derived sterile 20-like kinase
• Protein kinase lysine-deficient 1
• Protein kinase with no lysine 1
• protein kinase, lysine deficient 1
• serine/threonine-protein kinase WNK1
• WNK lysine deficient protein kinase 1
• WNK1

Background

WNK lysine deficient protein kinase 1 (WNK1) is a cytoplasmic serine-threonine kinase expressed in the distal nephron which likely plays an important role in hypertension. WNK1 is responsible for controling Na+ and Cl- ion transport by inhibiting the activity of other related protein kinases, such as WNK4.

WNK1 mutations have been associated with Gordon hyperkalemia-hypertension syndrome (pseudohypoaldosteronism type II) and congenital sensory neuropathy.

WNK1 is also known as Human Accelerated Region 5, and is thought to have played a key role in differentiating humans from apes.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for WNK1 Antibody (NBP2-75712)(1)

Publication using NBP2-75712

• Saha B, Dellova DCAL, Demko J et al. WNK1 is a chloride-stimulated scaffold that regulates mTORC2-activity and ion transport Journal of cell science 2022-11-14 [PMID: 36373794] (WB, Mouse)

Bioinformatics

• Gene Symbol: WNK1