Human WISP-1/CCN4 Recombinant Protein

Product Discontinued

Human WISP-1/CCN4 Recombinant Protein Summary

• Description: A single glycosylated polypeptide protein with a C-terminal 10x poly-His tag corresponding to amino acids Thr23 - Asn367 of WISP1 (O95388).
  
  

  Source: Mouse myeloma cell line, NS0

• Preparation Method: Lyophilized from 0.2 mm filtered concentrated solution in PBS.
• Details of Functionality: The ED50, as determined by a cell proliferation assay using NRK49F cells, is less than 7.5 mg/mL.
• Source: Nso cells
• Protein/Peptide Type: Recombinant Protein
• Gene: CCN4
• Purity: SDS-PAGE
• Endotoxin Note: Less than 1 EU/mg of the rHuWISP-1 as determined by LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
  • Functional
  • SDS-Page

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Concentration: LYOPH
• Purity: SDS-PAGE
• Reconstitution Instructions: Centrifuge prior to opening. Reconstitute with PBS to a final concentration of 0.1 mg/ml. Further dilutions should be made in appropriately buffered solutions.

Notes

This lyophilized preparation is stable at 2-8 degrees C, but should be kept at -20 degrees C for long term storage, preferably desiccated. Upon reconstitution, the preparation is most stable at -20 to -80 degrees C, and can be stored for one week at 2-8 degrees C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 degrees C to -80 degrees C. Avoid repeated freeze/thaw cycles.

Alternate Names for Human WISP-1/CCN4 Recombinant Protein

• CCN family member 4
• CCN4
• CCN4WISP1tc
• WISP1
• WISP-1
• WISP1c
• WISP1i
• WNT1 induced secreted protein 1
• WNT1 inducible signaling pathway protein 1
• Wnt-1 inducible signaling pathway protein 1
• wnt-1 signaling pathway protein 1
• Wnt-1-induced secreted protein
• WNT1-inducible-signaling pathway protein 1

Background

WISP-1 is a 40 kDa secreted, heparin-binding glycoprotein in the CCN cysteine-rich protein family and can associate into a homodimer. Multiple isoforms are generated by alternative splicing. WISP-1 is expressed by osteoblasts and may contribute to fracture healing by promoting bone cell formation.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: CCN4