WISP-1/CCN4 Overexpression Lysate

Images

 
Western Blot: WISP1 Overexpression Lysate (Adult Normal) [NBL1-17857] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for WISP1.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB

Order Details

WISP-1/CCN4 Overexpression Lysate Summary

Description

WISP-1/CCN4 Transient Overexpression Lysate


Expression Host: HEK293T

Plasmid: RC210402

Accession#: NM_003882

Protein Tag: C-MYC/DDK
Gene
CCN4

Applications/Dilutions

Dilutions
  • Western Blot
Application Notes
This product is intended for use as a positive control in Western Blot.

You will receive 1 vial of lysate (100ug), 1 vial of empty vector negative control (100ug), and 1 vial of 2xSDS sample buffer (250ul).
Theoretical MW
38 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
RIPA buffer

Lysate Details for Array

Type
Overexpression

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for WISP-1/CCN4 Overexpression Lysate

  • CCN family member 4
  • CCN4
  • CCN4WISP1tc
  • WISP1
  • WISP-1
  • WISP1c
  • WISP1i
  • WNT1 induced secreted protein 1
  • WNT1 inducible signaling pathway protein 1
  • Wnt-1 inducible signaling pathway protein 1
  • wnt-1 signaling pathway protein 1
  • Wnt-1-induced secreted protein
  • WNT1-inducible-signaling pathway protein 1

Background

This gene encodes a member of the WNT1 inducible signaling pathway (WISP) protein subfamily, which belongs to the connective tissue growth factor (CTGF) family. WNT1 is a member of a family of cysteine-rich, glycosylated signaling proteins that mediate diverse developmental processes. The CTGF family members are characterized by four conserved cysteine-rich domains: insulin-like growth factor-binding domain, von Willebrand factor type C module, thrombospondin domain and C-terminal cystine knot-like domain. This gene may be downstream in the WNT1 signaling pathway that is relevant to malignant transformation. It is expressed at a high level in fibroblast cells, and overexpressed in colon tumors. The encoded protein binds to decorin and biglycan, two members of a family of small leucine-rich proteoglycans present in the extracellular matrix of connective tissue, and possibly prevents the inhibitory activity of decorin and biglycan in tumor cell proliferation. It also attenuates p53-mediated apoptosis in response to DNA damage through activation of the Akt kinase. It is 83% identical to the mouse protein at the amino acid level. Alternative splicing of this gene generates 2 transcript variants. [provided by RefSeq]. Transcript Variant: This variant (1) encodes the full length protein. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

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Bioinformatics

Gene Symbol CCN4