WISP-1/CCN4 Antibody [Alexa Fluor™ Plus 488]

• Reactivity: Hu
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Alexa Fluor Plus 488

WISP-1/CCN4 Antibody [Alexa Fluor™ Plus 488] Summary

• Specificity: Detects human WISP‑1/CCN4 in direct ELISAs. In direct ELISAs, approximately 50% cross-reactivity with recombinant mouse WISP-1 is observed and less than 1% cross-reactivity with recombiant human (rh) CTGF and rhNOV is observed.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for WISP-1/CCN4 Antibody [Alexa Fluor™ Plus 488]

• CCN family member 4
• CCN4
• CCN4WISP1tc
• WISP1
• WISP-1
• WISP1c
• WISP1i
• WNT1 induced secreted protein 1
• WNT1 inducible signaling pathway protein 1
• Wnt-1 inducible signaling pathway protein 1
• wnt-1 signaling pathway protein 1
• Wnt-1-induced secreted protein
• WNT1-inducible-signaling pathway protein 1

Background

Human WISP-1 (Wnt-induced secreted protein-1; also CNN4) is a 40 kDa, secreted, heparin-binding glycoprotein that is a member of the CCN (or CTGF/Cyr61/Nov) cysteine-rich protein family (1‑5). It is synthesized as a 367 aa precursor that contains a series of structural homology modules. Following a 22 amino acid (aa) signal sequence, there is a 68 aa IGFBP-like domain (aa 53‑120), a 57 aa von Willebrand factor type C (VWC) module (aa 126‑182), a 40 aa TSP type I domain (aa 220‑259) and a 75 aa, C-terminal cysteine knot motif (aa 273‑347). The VWC module is associated with protein-protein interaction, the TSP domain binds sulfated glycoconjugates, and the cysteine knot mediates dimerization and receptor binding (4). It is likely that WISP-1 normally circulates as an 80 kDa homodimer (2). At least five alternative splice forms are known for WISP-1. One is 30 kDa in size, 258 aa in length, and shows a substitution of a His for aa 95‑182. This removes the VWC domain (2, 6). A second isoform is 155 aa in length and shows a frameshift at Arg 117 with a unique 38 aa C‑terminal extension. A third is 195 aa in length and shows a 31 aa substitution for the first 203 aa of the full length precursor (6). This retains the VWC and cysteine knot domains. A fourth shows a 43 aa substitution for aa 117‑367 for a total length of 163 aa. This effectively removes everything but the IGFBP-like domain (7). The last splice form contains a deletion of aa 25‑269 for a total length of 122 aa. Thus, only the signal sequence and cysteine knot motifs are retained (8). This leaves only the IGFBP-like domain (9). Full-length mature human WISP-1 is 85% aa identical to both mouse and rat WISP-1. WISP-1 is expressed by osteoblasts and may contribute to fracture healing by promoting bone cell formation (10, 11).

1. Pennica, D. et al. (1998) Proc. Natl. Acad. Sci. USA 95:14717.
2. Tanada, S. et al. (2001) Oncogene 20:5525.
3. Brigstock, D.R. et al. (2003) J. Clin. Pathol. Mol. Pathol. 56:127.
4. Desnoyers, L. (2004) Curr. Pharm. Des. 10:3913.
5. Brigstock, D.R. (2003) J. Endocrinol. 178:169.
6. Li, Z. (2005) GenBank Accession # AAP43925.
7. Li, Z. (2005) GenBank Accession # AAP43926.
8. Li, Z. (2005) GenBank Accession # AAP43924.
9. Cervello, M. et al. (2004) Ann. N.Y. Acad. Sci. 1028:432.
10. French, D.M. et al. (2004), Am. J. Pathol. 165:855.
11. Parisi, M.S. et al. (2006) Bone 38:671.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.