WISP-1/CCN4 Antibody (214203) [Alexa Fluor® 750]

• Reactivity: Mu
• Applications: WB
• Clone: 214203
• Clonality: Monoclonal
• Host: Rat
• Conjugate: Alexa Fluor 750

WISP-1/CCN4 Antibody (214203) [Alexa Fluor® 750] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant mouse WISP-1/CCN4
  Leu21-Asn367
  Accession # O54775
• Specificity: Detects mouse WISP-1/CCN4 in direct ELISAs and Western blots. In direct ELISAs and Western blots, this antibody shows approximately 10-30% cross-reactivity with recombinant human (rh) WISP-1 but no cross-reactivity with rhCTGF or rmNOV.
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Rat
• Purity Statement: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Western Blot

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for WISP-1/CCN4 Antibody (214203) [Alexa Fluor® 750]

• CCN family member 4
• CCN4
• CCN4WISP1tc
• WISP1
• WISP-1
• WISP1c
• WISP1i
• WNT1 induced secreted protein 1
• WNT1 inducible signaling pathway protein 1
• Wnt-1 inducible signaling pathway protein 1
• wnt-1 signaling pathway protein 1
• Wnt-1-induced secreted protein
• WNT1-inducible-signaling pathway protein 1

Background

Mouse WISP-1 (Wnt-induced secreted protein-1; also named CNN4) is a 40 kDa, secreted, heparin-binding glycoprotein that is a member of the CCN (or CTGF/Cyr61/Nov) cysteine-rich protein family (1- 6). It is synthesized as a 367 amino acid (aa) precursor that contains a series of structural homology modules. Following a 22 aa signal sequence, there is a 68 aa IGFBP-like domain (aa 53-120), a 66 aa von Willebrand factor type C (VWC) module (aa 121-186), a 41 aa TSP type I domain (aa 220-260) and a 75 aa, C-terminal cysteine knot motif (aa 273-347) (1, 6). The VWC module is associated with protein-protein interaction, the TSP domain binds sulfated glycoconjugates, and the cysteine knot mediates dimerization and receptor binding (4). It is likely that WISP-1 normally circulates as an 80 kDa homodimer (2). Multiple splice forms are reported for human WISP-1, but none for rodent (7-9). Mature mouse WISP-1 is 98%, 84%, and 85% aa identical to rat, canine, and human WISP-1, respectively. WISP-1 is expressed by fibroblasts and osteoblasts, and may contribute to fracture healing by promoting bone cell formation (1, 10, 11).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.